Počet záznamů: 1

Transposons and satellite DNA: on the origin of the major satellite DNA family in the Chenopodium genome

SYSNO ASEP	0533877
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Transposons and satellite DNA: on the origin of the major satellite DNA family in the Chenopodium genome
Tvůrce(i)	Belyayev, Alexander (BU-J)_{RID, ORCID} Josefiová, Jiřina (BU-J) Jandová, Michaela (BU-J) Mahelka, Václav (BU-J)_{RID, ORCID} Krak, Karol (BU-J)_{RID, ORCID} Mandák, Bohumil (BU-J)_{RID, ORCID}
Číslo článku	20
Zdroj.dok.	Mobile DNA. - : BioMed Central - ISSN 1759-8753 Roč. 11, č. 1 (2020), s. 1-10
Poč.str.	10 s.
Jazyk dok.	eng - angličtina
Země vyd.	GB - Velká Británie
Klíč. slova	CACTA transposons ; satellite DNA ; Chenopodium
Vědní obor RIV	EF - Botanika
Obor OECD	Plant sciences, botany
CEP	GA20-20286S GA ČR - Grantová agentura ČR
Způsob publikování	Open access
Institucionální podpora	BU-J - RVO:67985939
UT WOS	000545628600001
EID SCOPUS	85087526637
DOI	10.1186/s13100-020-00219-7
Anotace	Extensive and complex links exist between transposable elements (TEs) and satellite DNA (satDNA), which are the two largest fractions of eukaryotic genome. These relationships have a crucial effect on genome structure, function and evolution. Here, we report a novel case of mutual relationships between TEs and satDNA. In the genomes of Chenopodium s. str. species, the deletion derivatives of tnp2 conserved domain of the newly discovered CACTA-like TE Jozin are involved in generating monomers of the most abundant satDNA family of the Chenopodium satellitome. The analysis of the relative positions of satDNA and different TEs utilizing assembled Illumina reads revealed several associations between satDNA arrays and the transposases of putative CACTA-like elements when an 40 bp fragment of tnp2 served as the start monomer of the satDNA array. The high degree of identity of the consensus satDNA monomers of the investigated species and the tnp2 fragment (from 82.1 to 94.9%) provides evidence of the genesis of CficCI-61-40 satDNA family monomers from analogous regions of their respective parental elements. The results were confirmed via molecular genetic methods and Oxford Nanopore sequencing. The discovered phenomenon leads to the continuous replenishment of species genomes with new identical satDNA monomers, which in turn may increase species satellitomes similarity.
Pracoviště	Botanický ústav
Kontakt	Martina Bartošová, martina.bartosova@ibot.cas.cz, ibot@ibot.cas.cz, Tel.: 271 015 242 ; Marie Jakšová, marie.jaksova@ibot.cas.cz, Tel.: 384 721 156-8
Rok sběru	2021
Elektronická adresa	https://doi.org/10.1186/s13100-020-00219-7

Počet záznamů: 1