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Photobiochemical changes in Chlorella g120 culture during trophic conversion (metabolic pathway shift) from heterotrophic to phototrophic growth regime
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SYSNO ASEP 0533409 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Photobiochemical changes in Chlorella g120 culture during trophic conversion (metabolic pathway shift) from heterotrophic to phototrophic growth regime Tvůrce(i) Babaei, Azadeh (MBU-M) ORCID
Ranglová, Karolína (MBU-M) ORCID
Malapascua, José R.F. (MBU-M)
Torzillo, G. (IT)
Shayegan, J. (IR)
Silva Benavides, A. M. (CR)
Masojídek, Jiří (MBU-M) RID, ORCIDCelkový počet autorů 7 Zdroj.dok. Journal of Applied Phycology. - : Springer - ISSN 0921-8971
Roč. 32, č. 5 (2020), s. 2807-2818Poč.str. 12 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova Chlorella ; Chlorophyll fluorescence ; Heterotrophy ; Photosynthesis ; Respiration ; Trophic conversion Vědní obor RIV DA - Hydrologie a limnologie Obor OECD Marine biology, freshwater biology, limnology CEP LO1416 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Omezený přístup Institucionální podpora MBU-M - RVO:61388971 UT WOS 000539524100001 EID SCOPUS 85086317714 DOI 10.1007/s10811-020-02137-w Anotace Physiological and photobiochemical changes and growth in the heterotrophic strainChlorella vulgarisg120 were studied during trophic conversion from heterotrophic to phototrophic growth regime. After the exposure of theChlorellag120 culture to light, it revealed a significant activity of the electron transport (450-700 mu mol e(-) m(-2) s(-1)as measured by chlorophyll fluorescence) and high PSII photochemical yieldF(v)/F(m)between 0.7 and 0.8. Fast fluorescence induction kinetics showed that PSII electron acceptors in the plastoquinone pool remained partly oxidized, indicating no downregulation of PSII electron transport. The data further revealed that high photobiochemical activity is lost in futile (protective) processes of non-photochemical quenching and respiration which indicate that surplus energy is dissipated in these processes. Pigment analysis showed low chlorophyll content 0.35-1.15% as compared with exclusively phototrophic strainChlorella vulgarisR-117. Nevertheless, the carotenoid content in g120 was relatively high 0.20-0.33% of dry weight which resulted in a considerably high ratio of carotenoid/chlorophyll 0.31-0.44. This strain probably does not possess the fully competent photosynthetic apparatus and can only partially adapt to phototrophy. We show that the heterotrophically grown g120 strain can undergo metabolic shift from heterotrophic to phototrophic growth regime. It might be an interesting strain from biotechnological point of view as a source of carotenoids, especially lutein. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2021 Elektronická adresa https://link.springer.com/article/10.1007/s10811-020-02137-w
Počet záznamů: 1