Počet záznamů: 1
Revealing the true morphological structure of macroporous soft hydrogels for tissue engineering
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SYSNO ASEP 0533170 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Revealing the true morphological structure of macroporous soft hydrogels for tissue engineering Tvůrce(i) Podhorská, Bohumila (UMCH-V) ORCID
Vetrík, Miroslav (UMCH-V) RID, ORCID
Chylíková Krumbholcová, Eva (UMCH-V) RID
Kománková, Lucie (UMCH-V) RID, ORCID
Rashedi Banafshehvaragh, Niloufar (UMCH-V)
Šlouf, Miroslav (UMCH-V) RID, ORCID
Dušková-Smrčková, Miroslava (UMCH-V) RID
Janoušková, Olga (UMCH-V) RID, SAI, ORCIDČíslo článku 6672 Zdroj.dok. Applied Sciences-Basel. - : MDPI
Roč. 10, č. 19 (2020), s. 1-17Poč.str. 17 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova hydrogel scaffolds ; laser scanning confocal microscopy ; scanning electron microscopy Vědní obor RIV CD - Makromolekulární chemie Obor OECD Polymer science CEP GA17-11140S GA ČR - Grantová agentura ČR GA17-08531S GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora UMCH-V - RVO:61389013 UT WOS 000586763400001 EID SCOPUS 85092456125 DOI 10.3390/app10196672 Anotace Macroporous hydrogel scaffolds based on poly [N-(2-hydroxypropyl) methacrylamide] are one of the widely studied biocompatible materials for tissue reparation and regeneration. This study investigated the morphological changes during hydrogel characterization which can significantly influence their future application. Three types of macroporous soft hydrogels differing in pore size were prepared. The macroporosity was achieved by the addition of sacrificial template particles of sodium chloride of various sizes (0–30, 30–50, and 50–90 µm) to the polymerizing mixture. The 3D structure of the hydrogels was then investigated by scanning electron microscopy (SEM) and laser scanning confocal microscopy (LSCM). The SEM was performed with specimens rapidly frozen to various temperatures, while non-frozen gels were visualized with LSCM. In comparison to LSCM, the SEM images revealed a significant alteration in the mean pore size and appearance of newly formed multiple connections between the pores, depending on the freezing conditions. Additionally, after freezing for SEM, the gel matrix between the pores and the fine pores collapsed. LSCM visualization aided the understanding of the dynamics of pore generation using sodium chloride, providing the direct observation of hydrogel scaffolds with the growing cells. Moreover, the reconstructed confocal z-stacks were a promising tool to quantify the swollen hydrogel volume reconstruction which is not possible with SEM. Pracoviště Ústav makromolekulární chemie Kontakt Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Rok sběru 2021 Elektronická adresa https://www.mdpi.com/2076-3417/10/19/6672
Počet záznamů: 1