Počet záznamů: 1
Functional analysis of phospholipase Dδ family in tobacco pollen tubes
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SYSNO ASEP 0532422 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Functional analysis of phospholipase Dδ family in tobacco pollen tubes Tvůrce(i) Pejchar, Přemysl (UEB-Q) RID, ORCID
Sekereš, Juraj (UEB-Q) RID, ORCID
Novotný, Ondřej (UEB-Q) ORCID
Žárský, Viktor (UEB-Q) RID, ORCID
Potocký, Martin (UEB-Q) RID, ORCIDCelkový počet autorů 5 Zdroj.dok. Plant Journal. - : Wiley - ISSN 0960-7412
Roč. 103, č. 1 (2020), s. 212-226Poč.str. 15 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova membrane trafficking ; Nicotiana tabacum ; phosphatidic acid ; phospholipase D ; pollen tube ; tip growth ; tobacco Vědní obor RIV ED - Fyziologie Obor OECD Cell biology CEP GA17-27477S GA ČR - Grantová agentura ČR GA19-21758S GA ČR - Grantová agentura ČR EF16_019/0000738 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LM2015062 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UEB-Q - RVO:61389030 UT WOS 000528067600001 EID SCOPUS 85083764367 DOI 10.1111/tpj.14720 Anotace Phosphatidic acid (PA), an important signalling and metabolic phospholipid, is predominantly localized in the subapical plasma membrane (PM) of growing pollen tubes. PA can be produced from structural phospholipids by phospholipase D (PLD), but the isoforms responsible for production of PM PA were not identified yet and their functional roles remain unknown. Following genome-wide bioinformatic analysis of the PLD family in tobacco, we focused on the pollen-overrepresented PLDδ class. Combining live-cell imaging, gene overexpression, lipid-binding and structural bioinformatics, we characterized five NtPLDδ isoforms. Distinct PLDδ isoforms preferentially localize to the cytoplasm or subapical PM. Using fluorescence recovery after photobleaching, domain deletion and swapping analyses we show that membrane-bound PLDδs are tightly bound to PM, primarily via the central catalytic domain. Overexpression analyses suggested isoform PLDδ3 as the most important member of the PLDδ subfamily active in pollen tubes. Moreover, only PLDδ3 shows significant constitutive PLD activity in vivo and, in turn, PA promotes binding of PLDδ3 to the PM. This forms a positive feedback loop leading to PA accumulation and the formation of massive PM invaginations. Tightly controlled production of PA generated by PLDδ3 at the PM is important for maintaining the balance between various membrane trafficking processes that are crucial for plant cell tip growth. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2021 Elektronická adresa http://doi.org/10.1111/tpj.14720
Počet záznamů: 1