Počet záznamů: 1
Microfluidic device for concentration and SERS-based detection of bacteria in drinking water
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SYSNO ASEP 0532403 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Microfluidic device for concentration and SERS-based detection of bacteria in drinking water Tvůrce(i) Krafft, B. (DE)
Týčová, Anna (UIACH-O) ORCID
Urban, R. D. (DE)
Dusny, Ch. (DE)
Belder, D. (DE)Celkový počet autorů 5 Zdroj.dok. Electrophoresis. - : Wiley - ISSN 0173-0835
Roč. 42, 1-2 (2021), s. 86-94Poč.str. 9 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova porous membranes ; microfluidics ; porous membrane ; surface-enhanced Raman spectrometry Vědní obor RIV CB - Analytická chemie, separace Obor OECD Analytical chemistry CEP GJ20-14069Y GA ČR - Grantová agentura ČR Způsob publikování Omezený přístup Institucionální podpora UIACH-O - RVO:68081715 UT WOS 000535060400001 EID SCOPUS 85085517630 DOI 10.1002/elps.202000048 Anotace There is a constant need for the development of easy-to-operate systems for the rapid and unambiguous identification of bacterial pathogens in drinking water without the requirement for time-consuming culture processes. In this study, we present a disposable and low-cost lab-on-a-chip device utilizing a nanoporous membrane, which connects two stacked perpendicular microfluidic channels. Whereas one of the channels supplies the sample, the second one attracts it by potential-driven forces. Surface-enhanced Raman spectrometry (SERS) is employed as a reliable detection method for bacteria identification. To gain the effect of surface enhancement, silver nanoparticles were added to the sample. The pores of the membrane act as a filter trapping the bodies of microorganisms as well as clusters of nanoparticles creating suitable conditions for sensitive SERS detection. Therein, we focused on the construction and characterization of the device performance. To demonstrate the functionality of the microfluidic chip, we analyzed common pathogens (Escherichia coli DH5 alpha and Pseudomonas taiwanensis VLB120) from spiked tap water using the optimized experimental parameters. The obtained results confirmed our system to be promising for the construction of a disposable optical platform for reliable and rapid pathogen detection which couples their electrokinetic concentration on the integrated nanoporous membrane with SERS detection. Pracoviště Ústav analytické chemie Kontakt Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Rok sběru 2022 Elektronická adresa http://hdl.handle.net/11104/0310907
Počet záznamů: 1