Počet záznamů: 1
Human decellularized and crosslinked pericardium coated with bioactive molecular assemblies
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SYSNO ASEP 0532330 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Human decellularized and crosslinked pericardium coated with bioactive molecular assemblies Tvůrce(i) Musílková, Jana (FGU-C) RID, ORCID
Filová, Elena (FGU-C) RID, ORCID
Pala, J. (CZ)
Matějka, Roman (FGU-C) RID, ORCID, SAI
Hadraba, Daniel (FGU-C) RID, ORCID, SAI
Vondrášek, David (FGU-C) ORCID, SAI
Kaplan, Ondřej (FGU-C) RID
Riedel, Tomáš (UMCH-V) RID, ORCID
Brynda, Eduard (UMCH-V) RID
Kučerová, Johanka (UMCH-V)
Koňařík, M. (CZ)
Lopot, F. (CZ)
Pirk, J. (CZ)
Bačáková, Lucie (FGU-C) RID, ORCIDČíslo článku 015008 Zdroj.dok. Biomedical Materials. - : Institute of Physics Publishing - ISSN 1748-6041
Roč. 15, č. 1 (2020)Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova fibronectin ; human pericardium ; genipin ; glutaraldehyde ; endotelial cells Vědní obor RIV FA - Kardiovaskulární nemoci vč. kardiochirurgie Obor OECD Cardiac and Cardiovascular systems Vědní obor RIV – spolupráce Ústav makromolekulární chemie - Makromolekulární chemie CEP NV15-29153A GA MZd - Ministerstvo zdravotnictví Způsob publikování Omezený přístup Institucionální podpora FGU-C - RVO:67985823 ; UMCH-V - RVO:61389013 UT WOS 000563778900006 EID SCOPUS 85076327091 DOI 10.1088/1748-605X/ab52db Anotace Decellularized human pericardium is under study as an allogenic material for cardiovascular applications. The effects of crosslinking on the mechanical properties of decellularized pericardium were determined with a uniaxial tensile test, and the effects of crosslinking on the collagen structure of decellularized pericardium were determined by multiphoton microscopy. The viability of human umbilical vein endothelial cells seeded on decellularized human pericardium and on pericardium strongly and weakly crosslinked with glutaraldehyde and with genipin was evaluated by means of an MTS assay. The viability of the cells, measured by their metabolic activity, decreased considerably when the pericardium was crosslinked with glutaraldehyde. Conversely, the cell viability increased when the pericardium was crosslinked with genipin. Coating both non-modified pericardium and crosslinked pericardium with a fibrin mesh or with a mesh containing attached heparin and/or fibronectin led to a significant increase in cell viability. The highest degree of viability was attained for samples that were weakly crosslinked with genipin and modified by means of a fibrin and fibronectin coating. The results indicate a method by which in vivo endothelialization of human cardiac allografts or xenografts could potentially be encouraged. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2021 Elektronická adresa https://doi.org/10.1088/1748-605X/ab52db
Počet záznamů: 1