Počet záznamů: 1
Transient protein expression in tobacco BY-2 plant cell packs using single and multi-cassette replicating vectors
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SYSNO ASEP 0531903 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Transient protein expression in tobacco BY-2 plant cell packs using single and multi-cassette replicating vectors Tvůrce(i) Pobořilová, Zuzana (UEB-Q) ORCID, RID
Plchová, Helena (UEB-Q) RID, ORCID
Čeřovská, Noemi (UEB-Q) RID, ORCID
Gunter, C. J. (ZA)
Hitzeroth, I. I. (ZA)
Rybicki, E. P. (ZA)
Moravec, Tomáš (UEB-Q) RID, ORCIDCelkový počet autorů 7 Zdroj.dok. Plant Cell Reports. - : Springer - ISSN 0721-7714
Roč. 39, č. 9 (2020), s. 1115-1127Poč.str. 13 s. Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova DsRed ; gfp ; Plant cell pack ; Plant expression vector ; Tobacco BY-2 cells ; Transient co-expression Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Biochemistry and molecular biology CEP GA15-10768S GA ČR - Grantová agentura ČR EF16_019/0000738 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy TG03010009 GA TA ČR - Technologická agentura ČR Způsob publikování Open access Institucionální podpora UEB-Q - RVO:61389030 UT WOS 000528435000001 EID SCOPUS 85084142930 DOI 10.1007/s00299-020-02544-w Anotace Key message: This is the first evidence that replicating vectors can be successfully used for transient protein expression in BY-2 plant cell packs.
Abstract: Transient recombinant protein expression in plants and recently also plant cell cultures are of increasing interest due to the speed, safety and scalability of the process. Currently, studies are focussing on the design of plant virus-derived vectors to achieve higher amounts of transiently expressed proteins in these systems. Here we designed and tested replicating single and multi-cassette vectors that combine elements for enhanced replication and hypertranslation, and assessed their ability to express and particularly co-express proteins by Agrobacterium-mediated transient expression in tobacco BY-2 plant cell packs. Substantial yields of green and red fluorescent proteins of up to ~ 700 ng/g fresh mass were detected in the plant cells along with position-dependent expression. This is the first evidence of the ability of replicating vectors to transiently express proteins in BY-2 plant cell packs.Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2021 Elektronická adresa http://doi.org/10.1007/s00299-020-02544-w
Počet záznamů: 1