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Peptide mass mapping in bioapatites isolated from animal bones
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SYSNO ASEP 0531743 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Peptide mass mapping in bioapatites isolated from animal bones Tvůrce(i) Šmrhová, T. (CZ)
Junková, P. (CZ)
Kučková, Š. (CZ)
Suchý, Tomáš (USMH-B) RID, ORCID, SAI
Šupová, Monika (USMH-B) RID, ORCID, SAIČíslo článku 32 Zdroj.dok. Journal of Materials Science-Materials in Medicine. - : Springer - ISSN 0957-4530
Roč. 31, č. 3 (2020)Poč.str. 11 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova Bioapatite ; Collagen ; Liquid chromatography with tandem mass spectrometry ; Peptide mass mapping ; Relative quantification Vědní obor RIV JH - Keramika, žáruvzdorné materiály a skla Obor OECD Medical engineering CEP NV15-25813A GA MZd - Ministerstvo zdravotnictví Způsob publikování Omezený přístup Institucionální podpora USMH-B - RVO:67985891 UT WOS 000521322700001 EID SCOPUS 85081173067 DOI 10.1007/s10856-020-06371-z Anotace Bioapatite ceramics produced from biogenic sources provide highly attractive materials for the preparation of artificial replacements since such materials are not only more easily accepted by living organisms, but bioapatite isolated from biowaste such as xenogeneous bones also provides a low-cost material. Nevertheless, the presence of organic compounds in the bioapatite may lead to a deterioration in its quality and may trigger an undesirable immune response. Therefore, procedures which ensure the elimination of organic compounds through bioapatite isolation are being subjected to intense investigation and the presence of remaining organic impurities is being determined through the application of various methods. Since current conclusions concerning the conditions suitable for the elimination of organic compounds remain ambiguous, we used the mass spectrometry-based proteomic approach in order to determine the presence of proteins or peptides in bioapatite samples treated under the most frequently employed conditions, i.e., the alkaline hydrothermal proces and calcination at 500 °C. Since we also investigated the presence of proteins or peptides in treated bioapatite particles of differing sizes, we discovered that both calcination and the size of the bioapatite particles constitute the main factors influencing the presence of proteins or peptides in bioapatite. In fact, while intact proteins were detected even in calcinated bioapatite consisting of particles >250 μm, no proteins were detected in the same material consisting of particles <40 μm. Therefore, we recommend the use of powdered bioapatite for the preparation of artificial replacements since it is, more effectively purified than apatite in the form of blocks. In addition, we observed that while alkaline hydrothermal treatment leads to the non-specific cleavage of proteins, it does not ensure the full degradation thereof. Pracoviště Ústav struktury a mechaniky hornin Kontakt Iva Švihálková, svihalkova@irsm.cas.cz, Tel.: 266 009 216 Rok sběru 2021 Elektronická adresa https://link.springer.com/article/10.1007/s10856-020-06371-z
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