Počet záznamů: 1
Influence of Protein Modification and Glycosylation in the Catalytic Hydrogen Evolution Reaction of Avidin and Neutravidin: An Electrochemical Analysis
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SYSNO ASEP 0525619 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Influence of Protein Modification and Glycosylation in the Catalytic Hydrogen Evolution Reaction of Avidin and Neutravidin: An Electrochemical Analysis Tvůrce(i) Izadi, Nasim (BFU-R) ORCID
Černocká, Hana (BFU-R) RID, ORCID
Trefulka, Mojmír (BFU-R) RID, ORCID
Ostatná, Veronika (BFU-R) RID, ORCIDCelkový počet autorů 4 Zdroj.dok. ChemPlusChem. - : Wiley - ISSN 2192-6506
Roč. 85, č. 6 (2020), s. 1347-1353Poč.str. 7 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova streptavidin ; acid ; binding ; mercury ; signals Vědní obor RIV CG - Elektrochemie Obor OECD Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis) CEP GA18-18154S GA ČR - Grantová agentura ČR Způsob publikování Omezený přístup Institucionální podpora BFU-R - RVO:68081707 UT WOS 000544057700029 EID SCOPUS 85086975479 DOI 10.1002/cplu.202000298 Anotace To investigate glycans' influence on the behavior of glycoproteins on charged surfaces, avidin and its nonglycosylated and neutralized version neutravidin were studied by label-free chronopotentiometric stripping (CPS) analysis and alternating current voltammetry combined with a mercury electrode. Despite neutravidin's and avidin's similar size and structure, their CPS responses differed due to the different amounts of catalytically active free amino groups of lysine and arginine residues. Acetylation of the proteins resulted in the suppression of their CPS responses by almost four times for avidin and by about 50 % for neutravidin, respectively. On the other hand, the presence of glycans in the acetylated avidin induced about 30 % higher chronopotentiometric response compared to the acetylated neutravidin. We suggest that the presence, size and composition of the glycans influenced the CPS signal due to differences in the orientation at a charged surface. The obtained results can be utilized in glycoprotein research. Pracoviště Biofyzikální ústav Kontakt Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Rok sběru 2021 Elektronická adresa https://chemistry-europe.onlinelibrary.wiley.com/doi/full/10.1002/cplu.202000298
Počet záznamů: 1