Počet záznamů: 1
Isolation, Genomic and Metabolomic Characterization of Streptomyces tendae VITAKN with Quorum Sensing Inhibitory Activity from Southern India
- 1.
SYSNO ASEP 0525195 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Isolation, Genomic and Metabolomic Characterization of Streptomyces tendae VITAKN with Quorum Sensing Inhibitory Activity from Southern India Tvůrce(i) Ishaque, N.M. (IN)
Burgsdorf, I. (IL)
Limlingan Malit, J.J. (CN)
Saha, Subhasish (MBU-M) ORCID
Teta, R. (IT)
Kannabiran, K. (IN)
Hrouzek, Pavel (MBU-M) ORCID
Steindler, L. (IL)
Costantino, V. (IT)
Saurav, Kumar (MBU-M) ORCIDČíslo článku 121 Zdroj.dok. Microorganisms. - : MDPI
Roč. 1, JAN (2020)Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova natural product ; actinobacteria ; quorum sensing inhibition (QSI) Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology CEP EF18_070/0010493 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GJ19-17868Y GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora MBU-M - RVO:61388971 UT WOS 000514650700004 EID SCOPUS 85079034551 DOI 10.3390/microorganisms8010121 Anotace Streptomyces are among the most promising genera in terms of production ability to biosynthesize a variety of bioactive secondary metabolites with pharmaceutical interest. Coinciding with the increase in genomic sequencing of these bacteria, mining of their genomes for biosynthetic gene clusters (BGCs) has become a routine component of natural product discovery. Herein, we describe the isolation and characterization of a Streptomyces tendae VITAKN with quorum sensing inhibitory (QSI) activity that was isolated from southern coastal part of India. The nearly complete genome consists of 8,621,231bp with a GC content of 72.2%. Sequence similarity networks of the BGCs detected from this strain against the Minimum Information about a Biosynthetic Gene Cluster (MIBiG) database and 3365 BGCs predicted by antiSMASH analysis of publicly available complete Streptomyces genomes were generated through the BiG-SCAPE-CORASON platform to evaluate its biosynthetic novelty. Crude extract analysis using high-performance liquid chromatography connected to high resolution tandem mass spectrometry (HPLC-HRMS/MS) and dereplication through the Global Natural Product Social Molecular Networking (GNPS) online workflow resulted in the identification of cyclic dipeptides (2, 5-diketopiperazines, DKPs) in the extract, which are known to possess QSI activity. Our results highlight the potential of genome mining coupled with LC-HRMS/MS and in silico tools (GNPS) as a valid approach for the discovery of novel QSI lead compounds. This study also provides the biosynthetic diversity of BGCs and an assessment of the predicted chemical space yet to be discovered. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2021 Elektronická adresa https://www.mdpi.com/2076-2607/8/1/121
Počet záznamů: 1