Počet záznamů: 1
Properties of Lipid Models of Lung Surfactant Containing Cholesterol and Oxidized Lipids: A Mixed Experimental and Computational Study
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SYSNO ASEP 0524869 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Properties of Lipid Models of Lung Surfactant Containing Cholesterol and Oxidized Lipids: A Mixed Experimental and Computational Study Tvůrce(i) Olžyńska, Agnieszka (UFCH-W) RID
Delcroix, Pauline (UFCH-W)
Dolejsova, T. (CZ)
Krzaczek, K. (PL)
Korchowiec, B. (PL)
Czogalla, A. (PL)
Cwiklik, Lukasz (UFCH-W) RID, ORCIDZdroj.dok. Langmuir. - : American Chemical Society - ISSN 0743-7463
Roč. 36, č. 4 (2020), s. 1023-1033Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova pulmonary surfactant ; dynamics ; membranes ; simulations ; monolayers ; calcium Vědní obor RIV CF - Fyzikální chemie a teoretická chemie Obor OECD Physical chemistry CEP GA17-06792S GA ČR - Grantová agentura ČR Způsob publikování Omezený přístup Institucionální podpora UFCH-W - RVO:61388955 UT WOS 000511509900024 EID SCOPUS 85079021004 DOI 10.1021/acs.langmuir.9b02469 Anotace We introduce and study a multicomponent lipid film mimicking lipid composition of the human lung surfactant. It consists of phospholipids with various lipid headgroups and tail saturation. Furthermore, it includes cholesterol and oxidized lipids. Langmuir trough and fluorescence microscopy experiments are combined with fully atomistic molecular dynamics simulations. The considered lipid mixtures form complex interfacial films with properties modulated by lateral compression. Cholesterol laterally condenses, and oxidized lipids laterally expand the films. Both types of molecules increase film miscibility. Oxidized lipids also alter the lipid-water interface enhancing film hydration. This effect can be partially reversed by cholesterol. Regarding presentation of different chemical moieties toward the aqueous subphase, the zwitterionic phosphatidylcholine groups dominate at the lipid-water interface, while both the negatively charged phosphatidylglycerol and hydroxyl group of cholesterol are less exposed. The investigated synthetic lipid-only mimic of the lung surfactant may serve as a basis for further studies involving nonlipid pulmonary surfactant components. Pracoviště Ústav fyzikální chemie J.Heyrovského Kontakt Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196 Rok sběru 2021 Elektronická adresa http://hdl.handle.net/11104/0309114
Počet záznamů: 1