Počet záznamů: 1
Proinflammatory Effect of Carbon-Based Nanomaterials: In Vitro Study on Stimulation of Inflammasome NLRP3 via Destabilisation of Lysosomes
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SYSNO ASEP 0524868 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Proinflammatory Effect of Carbon-Based Nanomaterials: In Vitro Study on Stimulation of Inflammasome NLRP3 via Destabilisation of Lysosomes Tvůrce(i) Švadláková, T. (CZ)
Hubatka, F. (CZ)
Knötigová, P.T. (CZ)
Kulich, P. (CZ)
Mašek, J. (CZ)
Kotouček, J. (CZ)
Macák, J. (CZ)
Motola, M. (CZ)
Kalbáč, Martin (UFCH-W) RID, ORCID
Koláčková, M. (CZ)
Vaňková, R. (CZ)
Vicherková, P. (CZ)
Málková, A. (CZ)
Šimečková, P. (CZ)
Volkov, Y. (RU)
Prina-Mello, A. (IE)
Kratochvílová, Irena (FZU-D) RID, ORCID, SAI
Fiala, Z. (CZ)
Raška, M. (CZ)
Krejsek, J. (CZ)
Turánek, J. (CZ)Číslo článku 418 Zdroj.dok. Nanomaterials. - : MDPI
Roč. 10, č. 3 (2020)Poč.str. 18 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova functionalized graphene oxide ; pristine graphene ; oxidative stress ; nanoplatelets ; nanotubes ; toxicity ; release ; single ; system ; inflammogenicity ; graphene platelets ; carbon nanotubes ; inflammasome NLRP3 ; cathepsin B ; macrophages ; thp-1 Vědní obor RIV CF - Fyzikální chemie a teoretická chemie Obor OECD Physical chemistry Vědní obor RIV – spolupráce Fyzikální ústav - Biofyzika CEP EF16_019/0000760 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LM2015088 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy EF16_013/0001821 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UFCH-W - RVO:61388955 ; FZU-D - RVO:68378271 UT WOS 000526090400018 EID SCOPUS 85080938878 DOI 10.3390/nano10030418 Anotace Carbon-based nanomaterials (C-BNM) have recently attracted an increased attention as the materials with potential applications in industry and medicine. Bioresistance and proinflammatory potential of C-BNM is the main obstacle for their medicinal application which was documented in vivo and in vitro. However, there are still limited data especially on graphene derivatives such as graphene platelets (GP). In this work, we compared multi-walled carbon nanotubes (MWCNT) and two different types of pristine GP in their potential to activate inflammasome NLRP3 (The nod-like receptor family pyrin domain containing 3) in vitro. Our study is focused on exposure of THP-1/THP1-null cells and peripheral blood monocytes to C-BNM as representative models of canonical and alternative pathways, respectively. Although all nanomaterials were extensively accumulated in the cytoplasm, increasing doses of all C-BNM did not lead to cell death. We observed direct activation of NLRP3 via destabilization of lysosomes and release of cathepsin B into cytoplasm only in the case of MWCNTs. Direct activation of NLRP3 by both GP was statistically insignificant but could be induced by synergic action with muramyl dipeptide (MDP), as a representative molecule of the family of pathogen-associated molecular patterns (PAMPs). This study demonstrates a possible proinflammatory potential of GP and MWCNT acting through NLRP3 activation. Pracoviště Ústav fyzikální chemie J.Heyrovského Kontakt Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196 Rok sběru 2021 Elektronická adresa http://hdl.handle.net/11104/0309112
Počet záznamů: 1