Počet záznamů: 1
Selenium Incorporation to Amino Acids in Chlorella Cultures Grown in Phototrophic and Heterotrophic Regimes
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SYSNO ASEP 0524608 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Selenium Incorporation to Amino Acids in Chlorella Cultures Grown in Phototrophic and Heterotrophic Regimes Tvůrce(i) Mylenko, Mykola (MBU-M) ORCID
Vu, Dai Long (MBU-M) ORCID
Kuta, Jan (MBU-M) ORCID
Ranglová, Karolína (MBU-M) ORCID
Kubáč, David (MBU-M) RID, ORCID
Lakatos, Gergely Erno (MBU-M) ORCID
Grivalský, Tomáš (MBU-M) ORCID
Caporgano, Martin Pablo (MBU-M)
Manoel, Joao Camara (MBU-M) ORCID
Kopecký, Jiří (MBU-M) ORCID
Masojídek, Jiří (MBU-M) RID, ORCID
Hrouzek, Pavel (MBU-M) ORCIDZdroj.dok. Journal of Agricultural and Food Chemistry. - : American Chemical Society - ISSN 0021-8561
Roč. 68, č. 6 (2020), s. 1654-1665Poč.str. 12 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova Chlorella ; microalgae ; selenium Vědní obor RIV EI - Biotechnologie a bionika Obor OECD Agricultural biotechnology and food biotechnology CEP LO1416 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Výzkumná infrastruktura RECETOX II - 90051 - Masarykova univerzita Způsob publikování Omezený přístup Institucionální podpora MBU-M - RVO:61388971 UT WOS 000514256200016 EID SCOPUS 85079331947 DOI 10.1021/acs.jafc.9b06196 Anotace Microalgae accumulate bioavailable selenium-containing amino acids (Se-AAs), and these are useful as a food supplement. While this accumulation has been studied in phototrophic algal cultures, little data exists for heterotrophic cultures. We have determined the Se-AAs content, selenium/sulfur (Se/S) substitution rates, and overall Se accumulation balance in photo-and heterotrophic Chlorella cultures. Laboratory trials revealed that heterotrophic cultures tolerate Se doses, similar to 8-fold higher compared to phototrophic cultures, resulting in a similar to 2-3-fold higher Se-AAs content. In large-scale experiments, both cultivation regimes provided comparable Se-AAs content. Outdoor phototrophic cultures accumulated up to 400 mu g g(-1) of total Se-AAs and exhibited a high level of Se/S substitution (5-10%) with 30-60% organic/total Se embedded in the biomass. A slightly higher content of Se-AAs and ratio of Se/S substitution was obtained for a heterotrophic culture in pilot-scale fermentors. The data presented here shows that heterotrophic Chlorella cultures provide an alternative for Se-enriched biomass production and provides information on Se-AAs content and speciation in different cultivation regimes. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2021 Elektronická adresa https://pubs.acs.org/doi/full/10.1021/acs.jafc.9b06196
Počet záznamů: 1