Počet záznamů: 1

Chiroptical Properties and Conformation of Four Lasiocepsin-Related Antimicrobial Peptides: Structural Role of Disulfide Bridges

SYSNO ASEP	0524535
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Chiroptical Properties and Conformation of Four Lasiocepsin-Related Antimicrobial Peptides: Structural Role of Disulfide Bridges
Tvůrce(i)	Pazderková, Markéta (UOCHB-X)_{RID, ORCID} Profant, V. (CZ) Maloň, P. (CZ) Dukor, R. K. (US) Čeřovský, Václav (UOCHB-X)_{RID, ORCID} Baumruk, V. (CZ) Bednárová, Lucie (UOCHB-X)_{RID, ORCID}
Číslo článku	812
Zdroj.dok.	Symmetry-Basel. - : MDPI Roč. 12, č. 5 (2020)
Poč.str.	22 s.
Jazyk dok.	eng - angličtina
Země vyd.	CH - Švýcarsko
Klíč. slova	antimicrobial peptides ; lasiocepsin ; electronic circular dichroism ; vibrational circular dichroism ; Raman optical activity ; disulfide group
Vědní obor RIV	BO - Biofyzika
Obor OECD	Biophysics
CEP	GAP208/10/0376 GA ČR - Grantová agentura ČR
	GAP205/10/1276 GA ČR - Grantová agentura ČR
Způsob publikování	Open access
Institucionální podpora	UOCHB-X - RVO:61388963
UT WOS	000540226400130
EID SCOPUS	85085642389
DOI	10.3390/sym12050812
Anotace	We report an investigation of the role of disulfide bridges in the 27-residue antimicrobial peptide lasiocepsin (I) containing two disulfide groups (Cys8–Cys25, Cys17–Cys27) and three its analogs lacking one (II, III) or both (IV) native disulfides. Selective alternate incorporation of one or both disulfide bridges influences symmetry, conformation and biological properties of these peptides as demonstrated in their chiroptical (particularly Raman) properties. The effect of modifying the disulfide bridge pattern on the peptide secondary structure is investigated in water and in the presence of 2,2,2-trifluoroethanol and sodium dodecyl sulphate. A combination of experimental electronic and vibrational chiroptical data shows that both disulfide groups are necessary for stabilizing lasiocepsin secondary structure. While the Cys8–Cys25 disulfide group is important for sustaining lasiocepsin tertiary structure and maintaining its biological activity, the Cys17–Cys27 disulfide bridge has a supporting function consisting in reducing peptide flexibility.
Pracoviště	Ústav organické chemie a biochemie
Kontakt	asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434
Rok sběru	2021
Elektronická adresa	https://www.mdpi.com/2073-8994/12/5/812

Počet záznamů: 1