Počet záznamů: 1
Sequence analysis and FISH mapping of four satellite DNA families among Cervidae
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SYSNO ASEP 0524478 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Sequence analysis and FISH mapping of four satellite DNA families among Cervidae Tvůrce(i) Vozdová, M. (CZ)
Kubíčková, S. (CZ)
Černohorská, H. (CZ)
Fröhlich, J. (CZ)
Martínková, Natália (UBO-W) RID, ORCID, SAI
Rubeš, J. (CZ)Celkový počet autorů 6 Číslo článku 584 Zdroj.dok. Genes. - : MDPI
Roč. 11, č. 5 (2020)Poč.str. 15 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova centromere ; cervid phylogeny ; FISH ; satellite DNA ; sequencing Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Genetics and heredity (medical genetics to be 3) Způsob publikování Open access Institucionální podpora UBO-W - RVO:68081766 UT WOS 000542276700119 EID SCOPUS 85085452278 DOI 10.3390/genes11050584 Anotace Centromeric and pericentromeric chromosome regions are occupied by satellite DNA. Satellite DNAs play essential roles in chromosome segregation, and, thanks to their extensive sequence variability, to some extent, they can also be used as phylogenetic markers. In this paper, we isolated and sequenced satellite DNA I-IV in 11 species of Cervidae. The obtained satellite DNA sequences and their chromosomal distribution were compared among the analysed representatives of cervid subfamilies Cervinae and Capreolinae. Only satI and satII sequences are probably present in all analysed species with high abundance. On the other hand, fluorescence in situ hybridisation (FISH) with satIII and satIV probes showed signals only in a part of the analysed species, indicating interspecies copy number variations. Several indices, including FISH patterns, the high guanine and cytosine (GC) content, and the presence of centromere protein B (CENP-B) binding motif, suggest that the satII DNA may represent the most important satellite DNA family that might be involved in the centromeric function in Cervidae. The absence or low intensity of satellite DNA FISH signals on biarmed chromosomes probably reflects the evolutionary reduction of heterochromatin following the formation of chromosome fusions. The phylogenetic trees constructed on the basis of the satellite I-IV DNA relationships generally support the present cervid taxonomy. Pracoviště Ústav biologie obratlovců Kontakt Hana Slabáková, slabakova@ivb.cz, Tel.: 543 422 524 Rok sběru 2021 Elektronická adresa https://www.mdpi.com/2073-4425/11/5/584/pdf
Počet záznamů: 1