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Separation of anaesthetic ketamine and its derivates in PAMAPTAC coated capillaries with tuneable counter-current electroosmotic flow
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SYSNO ASEP 0524392 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Separation of anaesthetic ketamine and its derivates in PAMAPTAC coated capillaries with tuneable counter-current electroosmotic flow Tvůrce(i) Tůma, P. (CZ)
Koval, Dušan (UOCHB-X) RID, ORCID
Sommerová, B. (CZ)
Vaculín, Š. (CZ)Číslo článku 121094 Zdroj.dok. Talanta. - : Elsevier - ISSN 0039-9140
Roč. 217, Sep (2020)Poč.str. 8 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova clinical analysis ; capillary electrophoresis ; coating ; contactless conductivity detection ; Peak master ; stacking Vědní obor RIV CA - Anorganická chemie Obor OECD Analytical chemistry CEP GA18-02597S GA ČR - Grantová agentura ČR Způsob publikování Omezený přístup Institucionální podpora UOCHB-X - RVO:61388963 UT WOS 000537880200084 EID SCOPUS 85084174760 DOI 10.1016/j.talanta.2020.121094 Anotace Capillary electrophoretic separation of ketamine, norketamine, hydroxynorketamine, and dehydronorketamine was performed in the counter-current regime under the influence of oppositely-directed electroosmotic flow. For this purpose, the fused silica capillaries were covalently coated with the poly(acrylamide-co-3-acrylamidopropyl trimethylammonium chloride) copolymer (PAMAPTAC). The content of the cationic monomer APTAC in the polymerization mixture varied in the range 0–6 mol. % and the generated electroosmotic flow increased continuously in the 0–20 · 10−9 m2V−1s−1 interval. Importantly, it resulted in improved electrophoretic resolution of ketamine/norketamine, which increased from 0.8 for neutral PAM coating (i.e. 0% PAMAPTAC) to 3.0 for 6% PAMAPTAC. The determination of ketamine and its derivates in rat serum was performed in a 4% PAMAPTAC capillary with an inner diameter of 25 μm. The separation was performed in a 500 mM aqueous solution of acetic acid (pH 2.3). The clinical sample was deproteinized by the addition of acetonitrile to the serum and a large volume of the treated sample was injected directly into the capillary. The achieved limit of detection ranged from 2.2 ng/mL for dehydronorketamine to 4.1 ng/mL for hydroxynorketamine, the intra-day repeatability was 1.0–1.5% for the migration time and 2.8–3.3% for the peak area. The developed methodology was employed for time monitoring of ketamines in rat serum after intra venous administration of low doses of anaesthetic at a level of 2 μg per g of body weight. Pracoviště Ústav organické chemie a biochemie Kontakt asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434 Rok sběru 2021 Elektronická adresa https://www.sciencedirect.com/science/article/pii/S0039914020303854?via%3Dihub
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