Počet záznamů: 1
Electrographic seizures induced by activation of ETA and ETB receptors following intrahippocampal infusion of endothelin-1 in immature rats occur by different mechanisms
- 1.
SYSNO ASEP 0524259 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Electrographic seizures induced by activation of ETA and ETB receptors following intrahippocampal infusion of endothelin-1 in immature rats occur by different mechanisms Tvůrce(i) Vondráková, Kateřina (FGU-C) RID, ORCID, SAI
Novotný, P. (CZ)
Kubová, Hana (FGU-C) RID, ORCID
Pošusta, Antonín (FGU-C) RID
Boron, J. (CZ)
Fáberová, Veronika (FGU-C) ORCID, RID
Fabene, P. F. (IT)
Burchfiel, J. (US)
Tsenov, Grygoriy (FGU-C) RID, ORCIDČíslo článku 113255 Zdroj.dok. Experimental Neurology. - : Elsevier - ISSN 0014-4886
Roč. 328, Jun (2020)Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova ischemia ; hippocampus ; immature rat ; endothelin ; microdialysis, infmammatory ; mass spektrometry ; glutamate ; GABA ; leukotrienes ; receptors ; EEG ; seizures Vědní obor RIV FH - Neurologie, neurochirurgie, neurovědy Obor OECD Neurosciences (including psychophysiology CEP GA18-09296S GA ČR - Grantová agentura ČR GA14-20613S GA ČR - Grantová agentura ČR Způsob publikování Omezený přístup Institucionální podpora FGU-C - RVO:67985823 UT WOS 000527888300009 EID SCOPUS 85080116749 DOI 10.1016/j.expneurol.2020.113255 Anotace Using microdialysis we to determine biomarkers associated with seizures in P12 rats following the intrahippocampal infusion of two different agents: (1) ET-1, which activates both the ETA and ETB receptors and causes focal ischemia and (2) Ala-ET-1, which selectively activates only the ETB receptor and does not cause ischemia.
Our results show that seizures associated with combined ETA and ETB receptor activation (and ischemia) have a different temporal distribution and microdialysis profile from seizures associated with ETB activation alone (and without ischemia). Seizures induced with ETA and ETB activation peak within the first hour after infusion and the microdialysis profile is characterized by a significant increase in the ratio of glutamic acid to GABA. By contrast,
seizures with activation of only the ETB receptor peak in the second hour after infusion and microdialysis shows a significant increase in the ratio of leukotriene B4 to prostaglandin E2.
These findings suggest that ischemia-related seizures in immature animals involve an imbalance of excitation and inhibition, while non-ischemia related seizures involve an inflammatory process resulting from an excess of leukotrienes.
Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2021 Elektronická adresa https://doi.org/10.1016/j.expneurol.2020.113255
Počet záznamů: 1