Počet záznamů: 1
Study of permeabilization of bacterial membrane by electrochemical methods
- 1.
SYSNO ASEP 0524215 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Study of permeabilization of bacterial membrane by electrochemical methods Tvůrce(i) Čierna, M. (CZ)
Naumowicz, M. (PL)
Birošová, L. (SK)
Krahulec, J. (SK)
Sokolová, Romana (UFCH-W) RID, ORCID, SAI
Kolivoška, Viliam (UFCH-W) RID, ORCID
Sebechlebská, Táňa (UFCH-W) SAI, ORCID
Kielar, F. (TH)
Gál, M. (SK)Číslo článku 113761 Zdroj.dok. Journal of Electroanalytical Chemistry. - : Elsevier - ISSN 1572-6657
Roč. 857, JAN 2020 (2020)Poč.str. 5 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova antimicrobial peptides ; escherichia-coli ; carbon nutrition ; evolution ; ll-37 ; Voltammetry ; Cathelicidin ; Permeabilization ; E. coli Vědní obor RIV CG - Elektrochemie Obor OECD Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis) CEP GA18-09848S GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora UFCH-W - RVO:61388955 UT WOS 000518494300029 EID SCOPUS 85076881612 DOI 10.1016/j.jelechem.2019.113761 Anotace Selected electrochemical methods for the determination of possible membrane permeabilization in two Escherichia coli bacterial strains were examined. Escherichia coli, a gram-negative rod-shaped bacterium, is an important component of the mammalian intestinal microbiota. However, some strains of this bacterium are pathogenic and can cause a variety of diseases. Partial aim of this work was to identify beta-galactosidase enzyme as one of the possible factors characterizing disintegration of bacterial membrane. To permeabilize the bacterial membrane, cathelicidin LL-37, the body's own antimicrobial peptide, and membrane degradation by microwave radiation were used. In the investigation of structural changes in the bacterial membrane, square-wave voltammetry and chronopotentiometric dissolution analysis in combination with the mercury drop electrode and the glassy carbon electrode were used. UV/VIS spectrophotometry was used to indirectly track changes in bacterial cell structure. (C) 2019 The Authors. Published by Elsevier B.V. Pracoviště Ústav fyzikální chemie J.Heyrovského Kontakt Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196 Rok sběru 2021 Elektronická adresa 10.1016/j.jelechem.2019.113761
Počet záznamů: 1