Počet záznamů: 1
Spinal parenchymal occupation by neural stem cells after subpial delivery in adult immunodeficient rats
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SYSNO ASEP 0523980 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Spinal parenchymal occupation by neural stem cells after subpial delivery in adult immunodeficient rats Tvůrce(i) Maršala, M. (US)
Kamizato, K. (US)
Tadokoro, T. (US)
Navarro, M. (US)
Juhás, Štefan (UZFG-Y) RID, ORCID
Juhásová, Jana (UZFG-Y) RID, ORCID
Maršala, S. (US)
Studenovská, Hana (UMCH-V) RID, ORCID
Proks, Vladimír (UMCH-V) RID, ORCID
Hazel, T. (US)
Johe, K. (US)
Kakinohana, M. (JP)
Driscoll, S. (US)
Glenn, T. (US)
Pfaff, S. (US)
Ciacci, J. (US)Zdroj.dok. Stem Cells Translational Medicine. - : Oxford University Press - ISSN 2157-6564
Roč. 9, č. 2 (2020), s. 177-188Poč.str. 12 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova glia limitans formation from grafted neural precursors ; human-specific mRNA sequencing ; immunodeficient rat ; neuraxial neural precursor migration ; subpial stem cell injection Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Cell biology Vědní obor RIV – spolupráce Ústav makromolekulární chemie - Makromolekulární chemie CEP LO1609 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GA18-04393S GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora UZFG-Y - RVO:67985904 ; UMCH-V - RVO:61389013 UT WOS 000500683300001 EID SCOPUS 85076210651 DOI 10.1002/sctm.19-0156 Anotace Neural precursor cells (NSCs) hold great potential to treat a variety of neurodegenerative diseases and injuries to the spinal cord. However, current delivery techniques require an invasive approach in which an injection needle is advanced into the spinal parenchyma to deliver cells of interest. As such, this approach is associated with an inherent risk of spinal injury, as well as a limited delivery of cells into multiple spinal segments. Here, we characterize the use of a novel cell delivery technique that employs single bolus cell injections into the spinal subpial space. In immunodeficient rats, two subpial injections of human NSCs were performed in the cervical and lumbar spinal cord, respectively. The survival, distribution, and phenotype of transplanted cells were assessed 6-8 months after injection. Immunofluorescence staining and mRNA sequencing analysis demonstrated a near-complete occupation of the spinal cord by injected cells, in which transplanted human NSCs (hNSCs) preferentially acquired glial phenotypes, expressing oligodendrocyte (Olig2, APC) or astrocyte (GFAP) markers. In the outermost layer of the spinal cord, injected hNSCs differentiated into glia limitans-forming astrocytes and expressed human-specific superoxide dismutase and laminin. All animals showed normal neurological function for the duration of the analysis. These data show that the subpial cell delivery technique is highly effective in populating the entire spinal cord with injected NSCs, and has a potential for clinical use in cell replacement therapies for the treatment of ALS, multiple sclerosis, or spinal cord injury. Pracoviště Ústav živočišné fyziologie a genetiky Kontakt Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Rok sběru 2021 Elektronická adresa https://stemcellsjournals.onlinelibrary.wiley.com/doi/full/10.1002/sctm.19-0156
Počet záznamů: 1