Počet záznamů: 1
Crucial Role of Microbiota in Experimental Psoriasis Revealed by a Gnotobiotic Mouse Model
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SYSNO ASEP 0523660 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Crucial Role of Microbiota in Experimental Psoriasis Revealed by a Gnotobiotic Mouse Model Tvůrce(i) Stehlíková, Z. (CZ)
Kostovčíková, Klára (UMG-J)
Kverka, Miloslav (UEM-P)
Rossmann, P. (CZ)
Dvorak, J. (CZ)
Novosádová, I. (CZ)
Kostovčík, M. (CZ)
Coufal, Š. (CZ)
Šrůtková, D. (CZ)
Procházková, P. (CZ)
Hudcovic, T. (CZ)
Kozáková, H. (CZ)
Stěpánková, R. (CZ)
Rob, F. (CZ)
Jůzlová, K. (CZ)
Hercogová, J. (CZ)
Tlaskalová-Hogenová, H. (CZ)
Zákostelská, Z. (CZ)Celkový počet autorů 18 Číslo článku 236 Zdroj.dok. Frontiers in Microbiology. - : Frontiers Research Foundation - ISSN 1664-302X
Roč. 10, Feb 21 (2019)Poč.str. 11 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova psoriasis ; antibiotics ; microbiota ; germ-free ; animal model ; imiquimod ; intestine ; skin Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Microbiology Způsob publikování Open access Institucionální podpora UMG-J - RVO:68378050 UT WOS 000459260100001 DOI 10.3389/fmicb.2019.00236 Anotace Psoriatic patients have altered microbiota, both in the intestine and on the skin. It is not clear, however, whether this is a cause or consequence of the disease. In this study, using an experimental mouse model of psoriasis induced by imiquimod (IMO), we show that oral treatment with a broad spectrum of antibiotics (MIX) or metronidazole (MET) alone mitigates the severity of skin inflammation through downregulation of Th17 immune response in conventional mice. Since some antibiotics, including MET, can influence immune system reactivity, we also evaluated the effect of MIX in the same model under germ-free (GF) conditions. GF mice treated with MET did not show milder signs of imiquimod-induced skin inflammation (IISI) which supports the conclusion that the therapeutic effect is mediated by changes in microbiota composition. Moreover, compared to controls, mice treated with MIX had a significantly higher abundance of the genus Lactobacillus in the intestine and on the skin. Mice treated with MET had a significantly higher abundance of the genera Bifidobacterium and Enterococcus both on the skin and in the intestine and of Parabacteroides distasonis in the intestine. Additionally, GF mice and mice monocolonized with either Lactobacillus plantarum or segmented filamentous bacteria (SFB) were more resistant to IISI than conventional mice. Interestingly, compared to GF mice, IMQ induced a higher degree of systemic Th17 activation in mice monocolonized with SFB but not with L. plantarum. The present findings provide evidence that intestinal and skin microbiota directly regulates IISI and emphasizes the importance of microbiota in the pathogenesis of psoriasis. Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2020 Elektronická adresa https://www.frontiersin.org/articles/10.3389/fmicb.2019.00236/full
Počet záznamů: 1