Počet záznamů: 1
Colloidally stable polypeptide‐based nanogel: study of enzyme‐mediated nanogelation in inverse miniemulsion
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SYSNO ASEP 0522680 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Colloidally stable polypeptide‐based nanogel: study of enzyme‐mediated nanogelation in inverse miniemulsion Tvůrce(i) Dvořáková, Jana (UMCH-V) RID, ORCID
Šálek, Petr (UMCH-V) RID, ORCID
Korecká, L. (CZ)
Pavlova, Ewa (UMCH-V) RID
Černoch, Peter (UMCH-V) RID, ORCID
Janoušková, Olga (UMCH-V) RID, SAI, ORCID
Koutníková, Barbora (UMCH-V)
Proks, Vladimír (UMCH-V) RID, ORCIDČíslo článku 48725 Zdroj.dok. Journal of Applied Polymer Science. - : Wiley - ISSN 0021-8995
Roč. 137, č. 21 (2020), s. 1-10Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova biocompatible ; biodegradable ; human plasma Vědní obor RIV EI - Biotechnologie a bionika Obor OECD Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials CEP GA18-03224S GA ČR - Grantová agentura ČR Způsob publikování Omezený přístup Institucionální podpora UMCH-V - RVO:61389013 UT WOS 000496555800001 EID SCOPUS 85075144046 DOI 10.1002/app.48725 Anotace The current work presents a pivotal study of the nanogelation of the linear poly(N5‐2‐hydroxypropyl‐L‐glutamine) polymer precursor containing tyramine (TYR) units in an inverse miniemulsion by horseradish peroxidase/H2O2‐mediated crosslinking. The effects of various nH2O2/nTYR ratios on the kinetics of nanogelation in the inverse miniemulsion and on the reaction time are investigated by linear sweep voltammetry, while the formation of dityramine crosslinking is explored by fluorescence spectroscopy. The study is completed using dynamic light scattering measurements, nanoparticle tracking analysis, and cryogenic transmission electron microscopy to acquire comprehensive information about the formed nanoparticulate systems. With the optimal ratio nH2O2/nTYR = 2, the strategy yields in the high‐quality ~ 130 nm poly(amino acid)‐based nanogel, which is prepared in 2 h. The nanogel is colloidally stable under different temperature and pH conditions for over 168 h. Moreover, the demonstrated nanogel is noncytotoxic for HeLa cells and human primary fibroblasts and is quickly enzymatically hydrolyzed into small fragments during a biodegradation study in human blood plasma. Pracoviště Ústav makromolekulární chemie Kontakt Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Rok sběru 2021 Elektronická adresa https://onlinelibrary.wiley.com/doi/full/10.1002/app.48725
Počet záznamů: 1