Počet záznamů: 1
Simultaneous voltammetric determination of free tryptophan, uric acid, xanthine and hypoxanthine in plasma and urine
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SYSNO ASEP 0521718 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Simultaneous voltammetric determination of free tryptophan, uric acid, xanthine and hypoxanthine in plasma and urine Tvůrce(i) Hasoň, Stanislav (BFU-R) RID, ORCID
Ostatná, Veronika (BFU-R) RID, ORCID
Fojta, Miroslav (BFU-R) RID, ORCIDCelkový počet autorů 3 Číslo článku 135132 Zdroj.dok. Electrochimica acta. - : Elsevier - ISSN 0013-4686
Roč. 329, JAN 2020 (2020)Poč.str. 10 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova boron-doped diamond ; pencil graphite ; amino-acids ; carbon electrodes ; electrochemical oxidation Vědní obor RIV CG - Elektrochemie Obor OECD Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis) CEP GA18-18154S GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora BFU-R - RVO:68081707 UT WOS 000498391900034 EID SCOPUS 85074152340 DOI 10.1016/j.electacta.2019.135132 Anotace We have designed an improved electrochemical analysis of clinical samples in bodily fluids. The method allows reliable identification of several coexisting substances (tryptophan, uric acid, xanthine and hypoxanthine) present on different physiological levels. We prepared in situ electrogenerated graphite oxides (GrO) directly on the surface of a graphite pencil electrode. GrO presence enhances electrooxidation signals of small aromatic molecules and can shift their oxidation potentials. The oxidation potentials of tryptophan and tyrosine in their free and protein-bound forms, and free xanthine are very close to each other on common carbon-based materials. We found that free tryptophan's oxidation signal is significantly more enhanced (about 11-times) than the free tyrosine signal (only negligibly in the given conditions). Distinguishing xanthine's oxidation signal from that of the free tryptophan is allowed by their approximately 60 mV separation, sufficient for parallel determination by signal deconvolution. Additionally, we show that proteins containing tyrosine and/or tryptophan behave as electro-inactive and do not interfere with free amino acid determination on the GrO-modified graphite electrodes, while they strongly influence all species' detection on other carbon-based materials. Determining all individual compounds was possible at corresponding physiological values after sole dilution of human plasma (urine) and direct measurement with no pre-separation steps. (C) 2019 Elsevier Ltd. All rights reserved. Pracoviště Biofyzikální ústav Kontakt Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Rok sběru 2021 Elektronická adresa http://apps.webofknowledge.com/InboundService.do?customersID=Alerting&mode=FullRecord&IsProductCode=Yes&product=WOS&Init=Yes&Func=Frame&DestFail=http%3A%2F%2Fwww.webofknowledge.com&action=retrieve&SrcApp=Alerting&SrcAuth=Alerting&SID=F1U16JK9pRLeJ9ufBLv&UT=WOS%3A000498391900034
Počet záznamů: 1