Počet záznamů: 1
Method of detection of analyte active forms and determination of the ability of substances to bind into analyte active sites
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SYSNO ASEP 0520784 Druh ASEP P - Patent Zařazení RIV P - Patent nebo jiný výsledek chráněný podle zvláštních právních předpisů Název Method of detection of analyte active forms and determination of the ability of substances to bind into analyte active sites Tvůrce(i) Navrátil, Václav (UOCHB-X) RID, ORCID
Šácha, Pavel (UOCHB-X) RID, ORCID
Schimer, Jiří (UOCHB-X) RID, ORCID
Konvalinka, Jan (UOCHB-X) RID, ORCID
Majer, Pavel (UOCHB-X)Rok vydání 2019 Využití jiným subj. A - Pro využití výsledku jiným subjektem je vždy nutné nabytí licence Lic. popl. A - Poskytovatel licence požaduje licenční poplatek Číslo pat.spisu CA2957286 Datum udělení 06.08.2019 Vlastník patentu Ústav organické chemie a biochemie AV ČR, v. v. i Kód vydavatele patentu CA001 - Canadian Intellectual Property Office (CIPO) Gatineau, Québec Využití B - Patent je využíván na základě uzavřené licenční smlouvy mezi vlastníkem a uživatelem Jazyk dok. eng - angličtina Klíč. slova DIANA ; inhibitor ; analyt Vědní obor RIV CE - Biochemie Obor OECD Biochemical research methods CEP LO1302 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy NV15-31379A GA MZd - Ministerstvo zdravotnictví Institucionální podpora UOCHB-X - RVO:61388963 Anotace The invention provides a method for detection of active form of analytes in a sample and/or for determination of ability of tested substances to bind to the active site of these analytes, comprising the following steps: a) analyte or group of analytes from the sample is immobilized on the surface of a solid carrier either by non-specific non-covalent adsorption or by covalent binding of surface functional groups of the analyte and corresponding functional groups of the solid carrier, or preferably via a binding molecule which is bound to the surface of the solid carrier before immobilization of the analyte or group of analytes and is capable of selectively binding the analyte or group of analytes contained in the sample during incubation of the solid carrier with the sample, b) analyte or group of analytes is incubated with a detection probe which binds selectively to the analyte or group of analytes via a compound for selective binding to the analyte active site, whereas the probe consists of a low molecular compound for selective binding to the analyte active site, an oligonucleotide tag, optionally with a covalently attached fluorophore, biotin or a chemical group, and a chemical linker covalently linking the compound for selective binding to the analyte active site and the oligonucleotide tag, c) then the solid carrier is washed to remove unbound detection probe, and subsequently, the amount of bound detection probe is determined, whereas this amount is directly proportional to the amount of the analyte or group of analytes in the sample. The described method has broad application in medicine. Given the exceptional sensitivity of only a few dozen molecules, it provides the ability to determine the protein markers in blood in a concentration yet undetectable. Pracoviště Ústav organické chemie a biochemie Kontakt asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434 Rok sběru 2020 Elektronická adresa https://worldwide.espacenet.com/publicationDetails/originalDocument?CC=CA&NR=2957286C&KC=C&FT=D&ND=4&date=20190806&DB=&locale=en_EP#
Počet záznamů: 1