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NMR metabolomics reveals effects of Cryptosporidium infections on host cell metabolome
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SYSNO ASEP 0520226 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název NMR metabolomics reveals effects of Cryptosporidium infections on host cell metabolome Tvůrce(i) Miller, C.N. (GB)
Panagos, C.G. (GB)
Mosedale, W.R.T. (GB)
Kváč, Martin (BC-A) RID, RID, ORCID
Howard, M.J. (GB)
Tsaousis, A.D. (CA)Celkový počet autorů 6 Číslo článku 13 Zdroj.dok. Gut Pathogens. - : BioMed Central - ISSN 1757-4749
Roč. 11, APR 3 2019 (2019)Poč.str. 12 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova acquired-immunodeficiency-syndrome ; energy-metabolism ; oxidative stress ; parvum ; taurine ; culture ; burden ; epidemiology ; apicomplexan ; excystation ; Cryptosporidiosis ; nmr ; Metabolomics ; colo-680n ; Taurine Vědní obor RIV GJ - Choroby a škůdci zvířat, veterinární medicína Obor OECD Veterinary science CEP GA15-01090S GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora BC-A - RVO:60077344 UT WOS 000463842900001 EID SCOPUS 85063929769 DOI 10.1186/s13099-019-0293-x Anotace BackgroundCryptosporidium is an important gut microbe whose contributions towards infant and immunocompromise patient mortality rates are steadily increasing. Over the last decade, we have seen the development of various tools and methods for studying Cryptosporidium infection and its interactions with their hosts. One area that is sorely overlooked is the effect infection has on host metabolic processes.ResultsUsing a H-1 nuclear magnetic resonance approach to metabolomics, we have explored the nature of the mouse gut metabolome as well as providing the first insight into the metabolome of an infected cell line. Statistical analysis and predictive modelling demonstrated new understandings of the effects of a Cryptosporidium infection, while verifying the presence of known metabolic changes. Of note is the potential contribution of host derived taurine to the diarrhoeal aspects of the disease previously attributed to a solely parasite-based alteration of the gut environment, in addition to other metabolites involved with host cell catabolism.ConclusionThis approach will spearhead our understanding of the Cryptosporidium-host metabolic exchange and provide novel targets for tackling this deadly parasite. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2020 Elektronická adresa https://gutpathogens.biomedcentral.com/track/pdf/10.1186/s13099-019-0293-x
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