Počet záznamů: 1
High Mobility Group Box 1 and TLR4 Signaling Pathway in Gnotobiotic Piglets Colonized/Infected with L. amylovorus, L. mucosae, E. coli Nissle 1917 and S. Typhimurium.
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SYSNO ASEP 0519835 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název High Mobility Group Box 1 and TLR4 Signaling Pathway in Gnotobiotic Piglets Colonized/Infected with L. amylovorus, L. mucosae, E. coli Nissle 1917 and S. Typhimurium. Tvůrce(i) Šplíchal, Igor (MBU-M) RID
Donovan, S.M. (US)
Jeništová, Věra (MBU-M)
Šplíchalová, Iva (UMG-J)
Salmonová, H. (CZ)
Vlková, E. (CZ)
Neužil Bunešová, V. (CZ)
Šinkora, Marek (MBU-M) RID, ORCID
Killer, Jiří (UZFG-Y) RID, ORCID
Skřivanová, E. (CZ)
Šplíchalová, Alla (MBU-M) RID, ORCIDČíslo článku 6294 Zdroj.dok. International Journal of Molecular Sciences. - : MDPI
Roč. 20, Č. 24 (2019)Poč.str. 20 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova Escherichia coli Nissle 1917 ; Lactobacillus amylovorus ; Lactobacillus mucosae ; Salmonella Typhimurium Vědní obor RIV CE - Biochemie Obor OECD Biochemistry and molecular biology Vědní obor RIV – spolupráce Ústav molekulární genetiky - Biochemie
Ústav živočišné fyziologie a genetiky - Mikrobiologie, virologieCEP GA13-08803S GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora MBU-M - RVO:61388971 ; UMG-J - RVO:68378050 ; UZFG-Y - RVO:67985904 UT WOS 000506840100187 EID SCOPUS 85076856092 DOI 10.3390/ijms20246294 Anotace High mobility group box 1 (HMGB1) is a DNA-binding nuclear protein that can be actively secreted by immune cells after different immune stimuli or passively released from cells undergoing necrosis. HMGB1 amplifies inflammation, and its hypersecretion contributes to multiple organ dysfunction syndrome and death. We tested possible immunomodulatory effect of commensal Lactobacillus amylovorus (LA), Lactobacillus mucosae (LM) or probiotic Escherichia coli Nissle 1917 (EcN) in infection of gnotobiotic piglets with Salmonella Typhimurium (ST). Transcription of HMGB1 and Toll-like receptors (TLR) 2, 4, and 9 and receptor for advanced glycation end products (RAGE), TLR4-related molecules (MD-2, CD14, and LBP), and adaptor proteins (MyD88 and TRIF) in the ileum and colon were measured by RT-qPCR. Expression of TLR4 and its related molecules were highly upregulated in the ST-infected intestine, which was suppressed by EcN, but not LA nor LM. In contrast, HMGB1 expression was unaffected by ST infection or commensal/probiotic administration. HMGB1 protein levels in the intestine measured by ELISA were increased in ST-infected piglets, but they were decreased by previous colonization with E. coli Nissle 1917 only. We conclude that the stability of HMGB1 mRNA expression in all piglet groups could show its importance for DNA transcription and physiological cell functions. The presence of HMGB1 protein in the intestinal lumen probably indicates cellular damage. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2020 Elektronická adresa https://www.mdpi.com/1422-0067/20/24/6294
Počet záznamů: 1