Počet záznamů: 1
Antibacterial and antifungal activity of defensins from the Australian paralysis tick, Ixodes holocyclus
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SYSNO ASEP 0518834 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Antibacterial and antifungal activity of defensins from the Australian paralysis tick, Ixodes holocyclus Tvůrce(i) Cabezas-Cruz, A. (FR)
Tonk, M. (DE)
Bleackley, M.R. (AU)
Valdés, James J. (BC-A) RID, ORCID
Barrero, R. A. (AU)
Hernández-Jarguín, A. (ES)
Moutailler, S. (FR)
Vilcinskas, A. (DE)
Richard-Forget, F. (FR)
Anderson, M.A. (DE)
Rodriguez-Valle, M. (AU)Celkový počet autorů 11 Číslo článku 101269 Zdroj.dok. Ticks and Tick-borne Diseases - ISSN 1877-959X
Roč. 10, č. 6 (2019)Poč.str. 9 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova antimicrobial peptides ; protein ; sequence ; gene ; identification ; prediction ; energetics ; mechanism ; evolution ; immunity ; Ticks ; Defensins ; Antibacterial activity ; Antifungal activity ; Ixodes holocyclus Vědní obor RIV FN - Epidemiologie, infek. nemoci a klin. imunologie Obor OECD Infectious Diseases Způsob publikování Omezený přístup Institucionální podpora BC-A - RVO:60077344 UT WOS 000484783700018 EID SCOPUS 85070885430 DOI 10.1016/j.ttbdis.2019.101269 Anotace Tick innate immunity involves humoral and cellular responses. Among the humoral effector molecules in ticks are the defensins which are a family of small peptides with a conserved gamma-core motif that is crucial for their antimicrobial activity. Defensin families have been identified in several hard and soft tick species. However, little is known about the presence and antimicrobial activity of defensins from the Australian paralysis tick Ixodes holocyclus. In this study the I. holocyclus transcriptome was searched for the presence of defensins. Unique and non-redundant defensin sequences were identified and designated as holosins 1 5. The antimicrobial activity of holosins 2 and 3 and of the predicted y-cores of holosins 1-4 (HoloTickCores 1-4), was assessed using Gram-negative and Gram-positive bacteria as well as the fungus Fusarium graminearum and the yeast Candida albicans. All holosins had molecular features that are conserved in other tick defensins. Furthermore holosins 2 and 3 were very active against the Gram-positive bacteria Staphylococcus aureus and Listeria grayi. Holosins 2 and 3 were also active against F. graminearum and C. albicans and 5 mu M of peptide abrogate the growth of these microorganisms. The activity of the synthetic gamma-cores was lower than that of the mature defensins apart from HoloTickCore 2 which had activity comparable to mature holosin 2 against the Gram-negative bacterium Escherichia coli. This study reveals the presence of a multigene defensin family in I. holocyclus with wide antimicrobial activity. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2020 Elektronická adresa https://www.sciencedirect.com/science/article/abs/pii/S1877959X19302183?via%3Dihub
Počet záznamů: 1