Počet záznamů: 1
In vivo fluorescent cercariae reveal the entry portals of Cardiocephaloides longicollis (Rudolphi, 1819) Dubois, 1982 (Strigeidae) into the gilthead seabream Sparus aurata L.
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SYSNO ASEP 0518823 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název In vivo fluorescent cercariae reveal the entry portals of Cardiocephaloides longicollis (Rudolphi, 1819) Dubois, 1982 (Strigeidae) into the gilthead seabream Sparus aurata L. Tvůrce(i) van Beest, Gabrielle Shira (BC-A) ORCID
Villar-Torres, M. (ES)
Antonio Raga, J. (ES)
Esteban Montero, F. (ES)
Born-Torrijos, Ana (BC-A) RID, ORCIDCelkový počet autorů 5 Číslo článku 92 Zdroj.dok. Parasites & Vectors. - : BioMed Central - ISSN 1756-3305
Roč. 12, MAR 12 2019 (2019)Poč.str. 10 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova diplostomum-spathaceum trematoda ; fish intermediate host ; ornithodiplostomum-ptychocheilus ; parasite transmission ; survival ; brain ; recognition ; penetration ; infectivity ; migration ; Cardiocephaloides longicollis ; Cercarial penetration pattern ; Cercarial survival and activity ; Metacercarial encystment ; Digenea Vědní obor RIV EG - Zoologie Obor OECD Zoology CEP GBP505/12/G112 GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora BC-A - RVO:60077344 UT WOS 000461285800006 EID SCOPUS 85062830680 DOI 10.1186/s13071-019-3351-9 Anotace BackgroundDespite their complex life-cycles involving various types of hosts and free-living stages, digenean trematodes are becoming recurrent model systems. The infection and penetration strategy of the larval stages, i.e. cercariae, into the fish host is poorly understood and information regarding their entry portals is not well-known for most species. Cardiocephaloides longicollis (Rudolphi, 1819) Dubois, 1982 (Digenea, Strigeidae) uses the gilthead seabream (Sparus aurata L.), an important marine fish in Mediterranean aquaculture, as a second intermediate host, where they encyst in the brain as metacercariae. Labelling the cercariae with in vivo fluorescent dyes helped us to track their entry into the fish, revealing the penetration pattern that C. longicollis uses to infect S. aurata.MethodsTwo different fluorescent dyes were used: carboxyfluorescein diacetate succinimidyl ester (CFSE) and Hoechst 33342 (NB). Three ascending concentrations of each dye were tested to detect any effect on labelled cercarial performance, by recording their survival for the first 5 h post-labelling (hpl) and 24 hpl, as well as their activity for 5 hpl. Labelled cercariae were used to track the penetration points into fish, and cercarial infectivity and later encystment were analysed by recording brain-encysted metacercariae in fish infected with labelled and control cercariae after 20 days of infection.ResultsAlthough the different dye concentrations showed diverse effects on both survival and activity, intermediate doses of CFSE did not show any short-term effect on survival, permitting a brighter and longer recognition of cercariae on the host body surface. Therefore, CFSE helped to determine the penetration points of C. longicollis into the fish, denoting their aggregation on the head, eye and gills region, as well as on the dorsal fin and the lower side. Only CFSE-labelled cercariae showed a decreased number of encysted metacercariae when compared to control.ConclusionsOur study suggests that CFSE is an adequate labelling method for short-term in vivo studies, whereas NB would better suit in vivo studies on long-term performance. Cardiocephaloides longicollis cercariae seem to be attracted to areas near to the brain or those that are likely to be connected to migration routes to neuronal canals. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2020 Elektronická adresa https://parasitesandvectors.biomedcentral.com/track/pdf/10.1186/s13071-019-3351-9
Počet záznamů: 1