Počet záznamů: 1

Complex insight on microanatomy of larval human broad tapeworm Dibothriocephalus latus (Cestoda: Diphyllobothriidea)

SYSNO ASEP	0518822
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Complex insight on microanatomy of larval human broad tapeworm Dibothriocephalus latus (Cestoda: Diphyllobothriidea)
Tvůrce(i)	Barčák, Daniel (BC-A)_ORCID Yoneva, Aneta (BC-A) Sehadová, Hana (BC-A)_{RID, ORCID} Oros, M. (SK) Gustinelli, A. (IT) Kuchta, Roman (BC-A)_{RID, ORCID}
Celkový počet autorů	6
Číslo článku	408
Zdroj.dok.	Parasites & Vectors. - : BioMed Central - ISSN 1756-3305 Roč. 12, č. 1 (2019)
Poč.str.	17 s.
Forma vydání	Online - E
Jazyk dok.	eng - angličtina
Země vyd.	GB - Velká Británie
Klíč. slova	caryophyllaeus-laticeps caryophyllidea ; functional-morphology ; spirometra-erinacei ; scanning-electron ; nervous-system ; frontal glands ; fine-structure ; ultrastructure ; dendriticum ; pseudophyllidea ; Ultrastructure ; Immunofluorescence ; Plerocercoids ; Microtriches ; Receptors ; Glands ; Protonephridia ; Cestoda
Vědní obor RIV	EA - Morfologické obory a cytologie
Obor OECD	Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology
Vědní obor RIV – spolupráce	Entomologický ústav (do r. 2005) - Morfologické obory a cytologie
CEP	GX19-28399X GA ČR - Grantová agentura ČR
	LM2015062 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
Způsob publikování	Open access
Institucionální podpora	BC-A - RVO:60077344
UT WOS	000482904800001
EID SCOPUS	85071234639
DOI	10.1186/s13071-019-3664-8
Anotace	Background In Europe, the tapeworm Dibothriocephalus latus (syn. Diphyllobothrium latum) is a well-known etiological agent of human diphyllobothriosis, which spreads by the consumption of raw fish flesh infected by plerocercoids (tapeworm's larval stage). However, the process of parasite establishment in both intermediate and definitive hosts is poorly understood. This study was targeted mainly on the scolex (anterior part) of the plerocercoid of this species, which facilitates penetration of the parasite in intermediate paratenic fish hosts, and subsequently its attachment to the intestine of the definitive host. Methods Plerocercoids were isolated from the musculature of European perch (Perca fluviatilis) caught in Italian alpine lakes. Parasites were examined using confocal microscopy, scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Immunofluorescence tagging was held on whole mount larvae. Results The organisation of the central and peripheral nervous system was captured in D. latus plerocercoids, including the ultrastructure of the nerve cells possessing large dense neurosecretory granules. Two types of nerve fibres run from the body surface toward the nerve plexus located in the parenchyma on each side of bothria. One type of these fibres was found to be serotoninergic and possessed large subtegumental nerve cell bodies. A well-developed gland apparatus, found throughout the plerocercoid parenchyma, produced heterogeneous granules with lucent core packed in a dense layer. Three different types of microtriches occurred on the scolex and body surface of plerocercoids of D. latus: (i) uncinate spinitriches, (ii) coniform spinitriches, and (iii) capilliform filitriches. Non-ciliated sensory receptors were observed between the distal cytoplasm of the tegument and the underlying musculature. Conclusions Confocal laser scanning microscopy and electron microscopy (SEM and TEM) showed the detailed microanatomy of the nervous system in the scolex of plerocercoids, and also several differences in the larval stages compared with adult D. latus. These features, i.e. well-developed glandular system and massive hook-shaped uncinate spinitriches, are thus probably required for plerocercoids inhabiting fish hosts and also for their post-infection attachment in the human intestine.
Pracoviště	Biologické centrum (od r. 2006)
Kontakt	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Rok sběru	2020
Elektronická adresa	https://parasitesandvectors.biomedcentral.com/track/pdf/10.1186/s13071-019-3664-8

Počet záznamů: 1