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Interaction of MOPS buffer with glass-ceramic scaffold: Effect of (PO4)(3-) ions in SBF on kinetics and morphology of formatted hydroxyapatite

SYSNO ASEP	0518626
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Interaction of MOPS buffer with glass-ceramic scaffold: Effect of (PO4)(3-) ions in SBF on kinetics and morphology of formatted hydroxyapatite
Tvůrce(i)	Horkavcová, D. (CZ) Rohanová, D. (CZ) Stříbný, A. (CZ) Schuhladen, K. (DE) Boccaccini, A. R. (DE) Bezdička, Petr (UACH-T)_{SAI, RID, ORCID}
Celkový počet autorů	6
Zdroj.dok.	Journal of Biomedical Materials Research. Part B. - : Wiley - ISSN 1552-4973 Roč. 108, č. 5 (2020), s. 1888-1896
Poč.str.	9 s.
Jazyk dok.	eng - angličtina
Země vyd.	US - Spojené státy americké
Klíč. slova	bioglass (R) ; glass-ceramics scaffold ; in vitro test ; MOPS buffer ; simulated body fluid
Vědní obor RIV	CA - Anorganická chemie
Obor OECD	Inorganic and nuclear chemistry
Způsob publikování	Open access
Institucionální podpora	UACH-T - RVO:61388980
UT WOS	000502699200001
EID SCOPUS	85076742241
DOI	10.1002/jbm.b.34530
Anotace	The international standard ISO 23317:2014 for the in vitro testing of inorganic biomaterials in simulated body fluid (SBF) uses TRIS buffer to maintain neutral pH. In our previous papers, we investigated the interaction of a glass-ceramic scaffold with TRIS and HEPES buffers. Both of them speeded up glass-ceramic dissolution and hydroxyapatite (HAp) precipitation, thereby demonstrating their unsuitability for the in vitro testing of highly reactive biomaterials. In this article, we tested MOPS buffer (3-[N-morpholino] propanesulfonic acid), another amino acid from the group of “Goods buffers“. A highly reactive glass-ceramic scaffold (derived from Bioglass (R)) was exposed to SBF under static-dynamic conditions for 13/15 days. The kinetics and morphology of the newly precipitated HAp were studied using two different concentrations of (PO4)(3-) ions in SBF. The pH value and the Si-IV, Ca2+, and (PO4)(3-) concentrations in the SBF leachate samples were measured every day (AAS, spectrophotometry). The glass-ceramic scaffold was monitored by SEM/EDS, XRD, WD-XRF, and BET before and after 1, 3, 7, 11, and 13/15 days of exposure. As in the case of TRIS and HEPES, the preferential dissolution of the glass-ceramic crystalline phase (Combeite) was observed, but less intensively. The lower concentration of (PO4)(3-) ions slowed down the kinetics of HAp precipitation, thereby causing the disintegration of the scaffold structure. This phenomenon shows that the HAp phase was predominately generated by the presence of (PO4)(3-) ions in the SBF, not in the glass-ceramic material. Irrespective of this, MOPS buffer is not suitable for the maintenance of pH in SBF.
Pracoviště	Ústav anorganické chemie
Kontakt	Jana Kroneislová, krone@iic.cas.cz, Tel.: 311 236 931
Rok sběru	2021
Elektronická adresa	http://hdl.handle.net/11104/0308792

Počet záznamů: 1