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Development of breeding methods for increased lipids accumulation by marine microalgae using fluorescence-activated cell sorting
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SYSNO ASEP 0518558 Druh ASEP A - Abstrakt Zařazení RIV Záznam nebyl označen do RIV Zařazení RIV Není vybrán druh dokumentu Název Development of breeding methods for increased lipids accumulation by marine microalgae using fluorescence-activated cell sorting Tvůrce(i) Búzová, Diana (UEK-B) SAI, ORCID, RID
Literáková, Petra (UEK-B) ORCID, RID, SAI
Fedorko, Jan (UEK-B) SAI, ORCID
Kaštánek, P. (CZ)
Červený, Jan (UEK-B) RID, ORCID, SAIZdroj.dok. Book of abstract: 9th European Symposium on Plant Lipids. - Marseille, 2019 / Beisson Fred ; Li-Beisson Yonghua
S. 52-53Poč.str. 1 s. Akce 9th European Symposium on Plant Lipids Datum konání 07.07.2019 - 10.07.2019 Místo konání Marseille Země FR - Francie Typ akce EUR Jazyk dok. eng - angličtina Země vyd. FR - Francie Klíč. slova breeding methods ; lipids accumulation ; fluorescence-activated cell sorting ; microalgae CEP TN01000048 GA TA ČR - Technologická agentura ČR LO1415 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LM2015055 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora UEK-B - RVO:86652079 Anotace Development of breeding methods for increased lipids accumulation by marine microalgae using fluorescence-activated cell sorting. The marine microorganism belonging to the family Thraustochytriidae are group of non-photosynthetic marine protists. These unicellular, heterotrophic eukaryotes are commonly found in seawater and sediments, with the highest abundance in nutrient-rich areas, such as mangrove forests. Thraustochytrids are reported to be efficient producers of polyunsaturated fatty acids (PUFA) including the n-3 series, such as docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA). These organisms are considered as one of the potential alternative sources of PUFA for commercial and industrial exploitation. We developed advanced cultivation strategy and breeding method, and applied them on one particular Thraustochytrid strain Schizochytrium AN4 for optimization of lipids accumulation. The breeding method aims for selection of the hyper producing subpopulation using high-throughput fluorescence-activated cell sorting (FACS) in combination with imaging flow cytometry (IFC) and multiparametric discrimination the cells with both high lipid content and metabolic activity. The method was tested for negative influence on post-sorting cell viability possibly caused by the sorting technique procedure (e.g. high pressure, laser beam exposure, electro-magnetic field, toxic effects of both fluorescent marker and fluidic system medium composition) with no effect compared to non-sorted control sample. Pracoviště Ústav výzkumu globální změny Kontakt Nikola Šviková, svikova.n@czechglobe.cz, Tel.: 511 192 268 Rok sběru 2020
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