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The effect of silymarin flavonolignans and their sulfated conjugates on platelet aggregation and blood vessels ex vivo
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SYSNO ASEP 0518345 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název The effect of silymarin flavonolignans and their sulfated conjugates on platelet aggregation and blood vessels ex vivo Tvůrce(i) Pourová, J. (CZ)
Applová, L. (CZ)
Macáková, K. (CZ)
Vopršalová, M. (CZ)
Migkos, T. (CZ)
Bentanachs, R. (CZ)
Biedermann, David (MBU-M) RID, ORCID
Petrásková, Lucie (MBU-M) ORCID
Tvrdý, V. (CZ)
Hrubša, M. (CZ)
Karlíčková, J. (CZ)
Křen, Vladimír (MBU-M) RID, ORCID
Valentová, Kateřina (MBU-M) RID, ORCID
Mladěnka, P. (CZ)Číslo článku 2286 Zdroj.dok. Nutrients. - : MDPI
Roč. 11, č. 10 (2019)Poč.str. 16 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova Silymarin ; Milk Thiste ; Flavonolignans Vědní obor RIV FA - Kardiovaskulární nemoci vč. kardiochirurgie Obor OECD Cardiac and Cardiovascular systems CEP GA18-00121S GA ČR - Grantová agentura ČR LTC18003 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LTC18071 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora MBU-M - RVO:61388971 UT WOS 000498227300027 EID SCOPUS 85072847662 DOI 10.3390/nu11102286 Anotace Silymarin is a traditional drug and food supplement employed for numerous liver disorders. The available studies indicate that its activities may be broader, in particular due to claimed benefits in some cardiovascular diseases, but the contributions of individual silymarin components are unclear. Therefore, we tested silymarin flavonolignans as pure diastereomers as well as their sulfated metabolites for potential vasorelaxant and antiplatelet effects in isolated rat aorta and in human blood, respectively. Eleven compounds from a panel of 17 tested exhibited a vasorelaxant effect, with half maximal effective concentrations (EC50) ranging from 20 to 100 µM, and some substances retained certain activity even in the range of hundreds of nM. Stereomers A were generally more potent as vasorelaxants than stereomers B. Interestingly, the most active compound was a metabolite-silychristin-19-O-sulfate. Although initial experiments showed that silybin, 2,3-dehydrosilybin, and 2,3-dehydrosilychristin were able to substantially block platelet aggregation, their effects were rapidly abolished with decreasing concentration, and were negligible at concentrations ≤100 µM. In conclusion, metabolites of silymarin flavonolignans seem to have biologically relevant vasodilatory properties, but the effect of silymarin components on platelets is low or negligible. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2020 Elektronická adresa https://www.mdpi.com/2072-6643/11/10/2286
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