Počet záznamů: 1
Determination of binding constants of multiple charged cyclodextrin complexes by ACE using uncorrected and ionic strength corrected actual mobilities of the species involved
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SYSNO ASEP 0517791 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Determination of binding constants of multiple charged cyclodextrin complexes by ACE using uncorrected and ionic strength corrected actual mobilities of the species involved Tvůrce(i) Sázelová, Petra (UOCHB-X) RID, ORCID
Koval, Dušan (UOCHB-X) RID, ORCID
Severa, Lukáš (UOCHB-X) RID, ORCID
Teplý, Filip (UOCHB-X) RID, ORCID
Vigh, G. (US)
Kašička, Václav (UOCHB-X) RID, ORCIDZdroj.dok. Electrophoresis. - : Wiley - ISSN 0173-0835
Roč. 41, 7/8 (2020), s. 523-535Poč.str. 13 s. Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova affinity capillary electrophoresis ; binding constant ; cyclodextrins ; electrophoretic mobility ; ionic strength Vědní obor RIV CB - Analytická chemie, separace Obor OECD Analytical chemistry CEP GA17-10832S GA ČR - Grantová agentura ČR GA18-02597S GA ČR - Grantová agentura ČR Způsob publikování Omezený přístup Institucionální podpora UOCHB-X - RVO:61388963 UT WOS 000500308100001 EID SCOPUS 85076163030 DOI 10.1002/elps.201900352 Anotace In this study, the apparent binding constants and limiting mobilities of the multiply charged complexes of the Δ− and Λ−enantiomers of Ru(II)‐ and Fe(II)‐polypyridyl associates ([Ru(2,2′‐bipyridine)3]2+, [Ru(1,10‐phenanthroline)3]2+, and [Fe(1,10‐phenanthroline)3]2+) with single‐isomer 2,3‐diacetylated‐6‐sulfated‐cyclodextrins (CDs) (12Ac‐6S‐α‐CD, 14Ac‐7S‐β‐CD, and 16Ac‐8S‐γ‐CD) were determined by ACE using uncorrected and ionic strength corrected actual mobilities of the species involved. Two limiting models were tested for the ionic strength correction of the actual mobilities based on an empirical relation for the ionic strength correction of multivalent ionic species. In model 1, the nominal values of the charge numbers (zS,nom) and analytical concentrations (cS,nom) of the above CD selectors in the BGEs were applied for calculation of the BGE ionic strength, as usual. In model 2, the CD selectors were considered as singly charged species (zS = −1) with |zS,nom|‐times higher concentrations in the BGE than their analytical concentrations (cS = |zS,nom| × cS,nom) in the calculation of the BGE ionic strength. In all three cases–with uncorrected actual mobilities as well as with actual mobilities corrected according to the two limiting models–the measured effective mobilities of the above enantiomers fit well the theoretical curves of their mobility dependences on the CD selectors concentrations in the BGE, with high average coefficients of determination (R2 = 0.9890–0.9995). Nevertheless, the best physico‐chemically meaningful values of the apparent binding constants and the limiting mobilities of the enantiomer‐CDs complexes with low RSDs were obtained using the actual mobilities of the species involved corrected according to model 2. Pracoviště Ústav organické chemie a biochemie Kontakt asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434 Rok sběru 2021 Elektronická adresa https://onlinelibrary.wiley.com/doi/full/10.1002/elps.201900352
Počet záznamů: 1