Počet záznamů: 1
Nuclear magnetic resonance investigation of water transport through the plasma membrane of various yeast species
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SYSNO ASEP 0517572 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Nuclear magnetic resonance investigation of water transport through the plasma membrane of various yeast species Tvůrce(i) Šoltésová, M. (CZ)
Elicharová, Hana (FGU-C)
Srb, Pavel (UOCHB-X) RID, ORCID
Růžička, Michal (FGU-C)
Janisova, Larisa (UMCH-V) RID, ORCID
Sychrová, Hana (FGU-C) RID, ORCID
Lang, J. (CZ)Číslo článku fnz220 Zdroj.dok. FEMS Microbiology Letters. - : Oxford University Press - ISSN 0378-1097
Roč. 366, č. 18 (2019)Poč.str. 6 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova Saccharomyces cerevisiae ; water transport ; activation energy ; membrane permeability ; nuclear magnetic resonance spectroscopy ; yeast Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology Vědní obor RIV – spolupráce Ústav makromolekulární chemie - Biofyzika
Ústav organické chemie a biochemie - BiochemieCEP GA17-00973S GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora FGU-C - RVO:67985823 ; UMCH-V - RVO:61389013 ; UOCHB-X - RVO:61388963 UT WOS 000504086700003 EID SCOPUS 85075781156 DOI 10.1093/femsle/fnz220 Anotace A specific technique of nuclear magnetic resonance (NMR) spectroscopy, filter-exchange spectroscopy (FEXSY), was employed to investigate water transport through the plasma membrane in intact yeast cells. This technique allows water transport to be monitored directly, thus avoiding the necessity to subject the cells to any rapid change in the external conditions, e.g. osmotic shock. We established a sample preparation protocol, a data analysis procedure and verified the applicability of FEXSY experiments. We recorded the exchange rates in the temperature range 10–40°C for Saccharomyces cerevisiae. The resulting activation energy of 29 kJ mol−1 supports the hypothesis that water exchange is facilitated by water channels—aquaporins. Furthermore, we measured for the first time water exchange rates in three other phylogenetically unrelated yeast species (Schizosaccharomyces pombe, Candida albicans and Zygosaccharomyces rouxii) and observed remarkably different water exchange rates between these species. Findings of our work contribute to a better understanding of as fundamental a cell process as the control of water transport through the plasma membrane. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2020 Elektronická adresa https://doi.org/10.1093/femsle/fnz220
Počet záznamů: 1