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Silicalite-1 Layers as a Biocompatible Nano- and Micro-Structured Coating: An In Vitro Study on MG-63 Cells
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SYSNO ASEP 0517567 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Silicalite-1 Layers as a Biocompatible Nano- and Micro-Structured Coating: An In Vitro Study on MG-63 Cells Tvůrce(i) Doubková, Martina (FGU-C) ORCID, RID
Němčáková, Ivana (FGU-C) RID, ORCID
Jirka, Ivan (UFCH-W) RID, ORCID
Březina, V. (CZ)
Bačáková, Lucie (FGU-C) RID, ORCIDČíslo článku 3583 Zdroj.dok. Materials. - : MDPI
Roč. 12, č. 21 (2019)Poč.str. 13 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova silicalite-1 ; zeolite ; coating ; MG-63 cells ; osteoblasts ; biocompatibility Vědní obor RIV EI - Biotechnologie a bionika Obor OECD Biomaterials (as related to medical implants, devices, sensors) Vědní obor RIV – spolupráce Ústav fyzikální chemie J.Heyrovského - Fyzikální chemie a teoretická chemie CEP GA16-02681S GA ČR - Grantová agentura ČR GA19-02891S GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora FGU-C - RVO:67985823 ; UFCH-W - RVO:61388955 UT WOS 000502798800124 EID SCOPUS 85074658438 DOI 10.3390/ma12213583 Anotace Silicalite-1 is a purely siliceous form of zeolite, which does not contain potentially harmful aluminum in its structure as opposed to ZSM-5 aluminosilicate types of zeolite. This paper reports on a study of a silicalite-1 film, deposited on a silicon Si(100) substrate, as a potential anti-corrosive and biocompatible coating for orthopaedic implants. Silicalite-1 film was prepared in situ on the surface of Si(100) wafers using a reaction mixture of tetrapropyl-ammonium hydroxide (TPAOH), tetraethyl-orthosilicate (TEOS), and diH2O. The physico-chemical properties of the obtained surface were characterized by means of X-ray photoelectron spectroscopy, water contact angle measurement, atomic force microscopy, and scanning electron microscopy. The biocompatibility was assessed by interaction with the MG-63 cell line (human osteosarcoma) in terms of cell adhesion, morphology, proliferation, and viability. The synthesized silicalite-1 film consisted of two layers (b- and a, b-oriented crystals) creating a combination of micro- and nano-scale surface morphology suitable for cell growth. Despite its hydrophobicity, the silicalite-1 film increased the number of initially adhered human osteoblast-like MG-63 cells and the proliferation rate of these cells. The silicalite-1 film also improved the cell viability in comparison with the reference Si(100) substrate. It is therefore a promising candidate for coating of orthopaedic implants. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2020 Elektronická adresa https://doi.org/10.3390/ma12213583
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