Počet záznamů: 1
The effects of nano-sized PbO on biomarkers of membrane disruption and DNA damage in a sub-chronic inhalation study on mice
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SYSNO ASEP 0510415 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název The effects of nano-sized PbO on biomarkers of membrane disruption and DNA damage in a sub-chronic inhalation study on mice Tvůrce(i) Bláhová, L. (CZ)
Nováková, Z. (CZ)
Večeřa, Zbyněk (UIACH-O) RID
Vrlíková, Lucie (UZFG-Y) ORCID
Dočekal, Bohumil (UIACH-O) RID, ORCID
Dumková, J. (CZ)
Křůmal, Kamil (UIACH-O) RID, ORCID
Mikuška, Pavel (UIACH-O) RID, ORCID
Buchtová, Marcela (UZFG-Y) RID, ORCID
Hampl, A. (CZ)
Hilscherová, K. (CZ)
Bláha, L. (CZ)Celkový počet autorů 12 Zdroj.dok. Nanotoxicology. - : Taylor & Francis - ISSN 1743-5390
Roč. 14, č. 2 (2020), s. 214-231Poč.str. 18 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova environmental toxicology ; exposure ; mechanistic toxicology ; nanoparticles Vědní obor RIV CB - Analytická chemie, separace Obor OECD Analytical chemistry Vědní obor RIV – spolupráce Ústav živočišné fyziologie a genetiky - Genetika a molekulární biologie CEP GAP503/11/2315 GA ČR - Grantová agentura ČR GBP503/12/G147 GA ČR - Grantová agentura ČR Způsob publikování Omezený přístup Institucionální podpora UIACH-O - RVO:68081715 ; UZFG-Y - RVO:67985904 UT WOS 000496628900001 EID SCOPUS 85075138170 DOI 10.1080/17435390.2019.1685696 Anotace Although the production of engineered nanoparticles increases our knowledge of toxicity and mechanisms of bioactivity during relevant exposures is lacking. In the present study mice were exposed to PbO nanoparticles for 2, 5 and 13 weeks through continuous inhalation. The analyses addressed Pb and PbONP distribution in organs (lung, liver, kidney, brain) using electrothermal atomic absorption spectrometry and transmission electron microscopy, as well as histopathology and analyses of oxidative stress biomarkers. New LC-MS/MS methods were validated for biomarkers of
lipid damage F2-isoprostanes (8-iso-prostaglandins F2-alpha and E2) and hydroxylated deoxoguanosine (8-OHdG, marker of DNA oxidation). Commonly studied malondialdehyde was also measured as TBARS by HPLC-DAD. The study revealed fast blood transport and distribution of Pb from the lung to the kidney and liver. A different Pb accumulation trend was observed in the brain, suggesting transfer of NP along the nasal nerve to the olfactory bulbs. Long-term inhalation of PbONP caused lipid peroxidation in animal brains (increased levels of TBARS and both isoprostanes). Membrane lipid damage was also detected in the kidney
after shorter exposures, but not in the liver or lung. On the contrary, longer exposures to PbONP increased levels of 8-OHdG in the lung and temporarily increased lung weight after 2 and 5 weeks of exposure. The histopathological changes observed mainly in the lung and liver indicated inflammation and general toxicity responses. The present long-term inhalation study indicates risks of PbONP to both human health and the environment.Pracoviště Ústav analytické chemie Kontakt Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Rok sběru 2021 Elektronická adresa http://hdl.handle.net/11104/0303358
Počet záznamů: 1