Počet záznamů: 1
Pre-treatment and elution of amino acids from dried blood spots for direct determination by capillary electrophoresis
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SYSNO ASEP 0509088 Druh ASEP A - Abstrakt Zařazení RIV Záznam nebyl označen do RIV Zařazení RIV Není vybrán druh dokumentu Název Pre-treatment and elution of amino acids from dried blood spots for direct determination by capillary electrophoresis Tvůrce(i) Ryšavá, Lenka (UIACH-O) RID, ORCID, SAI
Dvořák, Miloš (UIACH-O)
Miková, B. (CZ)
Kubáň, Pavel (UIACH-O) RID, ORCIDCelkový počet autorů 4 Zdroj.dok. CECE 2019. - Gdańsk : University of Gdańsk, 2019
S. 20-20Poč.str. 1 s. Forma vydání Nosič - C Akce CECE 2019. International Interdisciplinary Meeting on Bioanalysis /16./ Datum konání 24.09.2019 - 26.09.2019 Místo konání Gdańsk Země PL - Polsko Typ akce WRD Jazyk dok. eng - angličtina Země vyd. PL - Polsko Klíč. slova amino acids ; capillary electrophoresis ; contactless conductivity detection Vědní obor RIV CB - Analytická chemie, separace Obor OECD Analytical chemistry CEP GA18-13135S GA ČR - Grantová agentura ČR Institucionální podpora UIACH-O - RVO:68081715 Anotace In this contribution, the process of sampling the DBSs and their subsequent elution with deionized water, methanol, acetonitrile, and optionally with mixtures thereof is shown. Various effects of the co-eluted matrix components on CE-C4D were observed for the selected elution solutions and are comprehensively described. The CE-C4D method was optimized for baseline separation of biogenic amino acids and indicated excellent analytical parameters and high stability of the separation system. CE-C4D was used for the direct analysis of the DBS eluates from healthy donors with amino acid concentrations at physiological levels. Finally, the method was applied to the determination of selected amino acids from DBS samples, which are related to common inborn metabolic disorders, such as maple syrup urine disease and phenylketonuria. Pracoviště Ústav analytické chemie Kontakt Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Rok sběru 2020
Počet záznamů: 1