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A pilot study of the UVA-photoprotective potential of dehydrosilybin, isosilybin, silychristin, and silydianin on human dermal fibroblasts
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SYSNO ASEP 0508426 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název A pilot study of the UVA-photoprotective potential of dehydrosilybin, isosilybin, silychristin, and silydianin on human dermal fibroblasts Tvůrce(i) Svobodová, A.R. (CZ)
Gabrielová, E. (CZ)
Ulrichová, J. (CZ)
Zálešák, B. (CZ)
Biedermann, David (MBU-M) RID, ORCID
Vostálová, J. (CZ)Zdroj.dok. Archives of Dermatological Research. - : Springer - ISSN 0340-3696
Roč. 311, č. 6 (2019), s. 477-490Poč.str. 14 s. Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova Cell culture ; Flavonolignan ; UVA Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology Způsob publikování Omezený přístup Institucionální podpora MBU-M - RVO:61388971 UT WOS 000473002100007 EID SCOPUS 85065730802 DOI 10.1007/s00403-019-01928-7 Anotace The exposure of naked unprotected skin to solar radiation may result in numerous acute and chronic undesirable effects. Evidence suggests that silymarin, a standardized extract from Silybum marianum (L.) Gaertn. seeds, and its major component silybin suppress UVB-induced skin damage. Here, we aimed to investigate the UVA-protective effects of silymarins less abundant flavonolignans, specifically isosilybin (ISB), silychristin (SC), silydianin (SD), and 2,3-dehydrosilybin (DHSB). Normal human dermal fibroblasts (NHDF) pre-treated for 1h with flavonolignans were then exposed to UVA light using a solar simulator. Their effects on reactive oxygen species (ROS), carbonylated proteins and glutathione (GSH) level, caspase-3 activity, single-strand breaks' (SSBs) formation and protein level of matrix metalloproteinase-1 (MMP-1), heme oxygenase-1 (HO-1), and heat shock protein (HSP70) were evaluated. The most pronounced preventative potential was found for DHSB, a minor component of silymarin, and SC, the second most abundant flavonolignan in silymarin. They had significant effects on most of the studied parameters. Meanwhile, a photoprotective effect of SC was mostly found at double the concentration of DHSB. ISB and SD protected against GSH depletion, the generation of ROS, carbonylated proteins and SSBs, and caspase-3 activation, but had no significant effect on MMP-1, HO-1, or HSP70. In summary, DHSB and to a lesser extent other silymarin flavonolignans are potent UVA-protective compounds. However, due to the in vitro phototoxic potential of DHSB published elsewhere, further studies are needed to exclude phototoxicity for humans as well as to confirm our results on human skin ex vivo and in vivo. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2020 Elektronická adresa https://link.springer.com/article/10.1007%2Fs00403-019-01928-7
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