Počet záznamů: 1
Reversible and Irreversible Modulation of Tubulin Self-Assembly by Intense Nanosecond Pulsed Electric Fields
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SYSNO ASEP 0507886 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Reversible and Irreversible Modulation of Tubulin Self-Assembly by Intense Nanosecond Pulsed Electric Fields Tvůrce(i) Chafai, Djamel Eddine (URE-Y)
Sulimenko, Vadym (UMG-J) RID, ORCID
Havelka, Daniel (URE-Y) RID
Kubínová, Lucie (FGU-C) RID, ORCID
Dráber, Pavel (UMG-J) RID, ORCID
Cifra, Michal (URE-Y) RID, ORCID, SAICelkový počet autorů 6 Číslo článku e1903636 Zdroj.dok. Advanced Materials. - : Wiley - ISSN 0935-9648
Roč. 31, č. 39 (2019)Poč.str. 7 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova nanosecond pulsed electric field ; microtubules ; self-assembly ; tubulin Vědní obor RIV JA - Elektronika a optoelektronika, elektrotechnika Obor OECD Electrical and electronic engineering Vědní obor RIV – spolupráce Ústav molekulární genetiky - Morfologické obory a cytologie
Fyziologický ústav - Morfologické obory a cytologieCEP GA17-11898S GA ČR - Grantová agentura ČR GA18-27197S GA ČR - Grantová agentura ČR GA18-23597S GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora URE-Y - RVO:67985882 ; UMG-J - RVO:68378050 ; FGU-C - RVO:67985823 UT WOS 000481222200001 EID SCOPUS 85070672049 DOI 10.1002/adma.201903636 Anotace Tubulin self-assembly into microtubules is a fascinating natural phenomenon. Its importance is not just crucial for functional and structural biological processes, but it also serves as an inspiration for synthetic nanomaterial innovations. The modulation of the tubulin self-assembly process without introducing additional chemical inhibitors/promoters or stabilizers has remained an elusive process. This work reports a versatile and vigorous strategy for controlling tubulin self-assembly by nanosecond electropulses (nsEPs). The polymerization assessed by turbidimetry is dependent on nsEPs dosage. The kinetics of microtubules formation is tightly linked to the nsEPs effects on structural properties of tubulin, and tubulin-solvent interface, assessed by autofluorescence, and the zeta potential. Moreover, the overall size of tubulin assessed by dynamic light scattering is affected as well. Additionally, atomic force microscopy imaging reveals the formation of different assemblies reflecting applied nsEPs. It is suggested that changes in C-terminal modification states alter tubulin polymerization-competent conformations. Although the assembled tubulin preserve their integral structure, they might exhibit a broad range of new properties important for their functions. Thus, these transient conformation changes of tubulin and their collective properties can result in new applications Pracoviště Ústav fotoniky a elektroniky Kontakt Petr Vacek, vacek@ufe.cz, Tel.: 266 773 413, 266 773 438, 266 773 488 Rok sběru 2020 Elektronická adresa https://onlinelibrary.wiley.com/doi/full/10.1002/adma.201903636
Počet záznamů: 1