Počet záznamů: 1
Activity and inactivity of moth sex chromosomes in somatic and meiotic cells
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SYSNO ASEP 0507648 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Activity and inactivity of moth sex chromosomes in somatic and meiotic cells Tvůrce(i) Traut, W. (DE)
Schubert, V. (DE)
Dalíková, Martina (BC-A) RID, ORCID
Marec, František (BC-A) RID, ORCID
Sahara, K. (JP)Celkový počet autorů 5 Zdroj.dok. Chromosoma. - : Springer - ISSN 0009-5915
Roč. 128, č. 4 (2019), s. 533-545Poč.str. 13 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova heterochromatin ; sex chromatin ; germline-limited activity Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Genetics and heredity (medical genetics to be 3) CEP GA17-13713S GA ČR - Grantová agentura ČR Způsob publikování Omezený přístup Institucionální podpora BC-A - RVO:60077344 UT WOS 000495294600003 EID SCOPUS 85070911671 DOI 10.1007/s00412-019-00722-8 Anotace Moths and butterflies (Lepidoptera) are the most species-rich group of animals with female heterogamety, females mostly having a WZ, males a ZZ sex chromosome constitution. We studied chromatin conformation, activity, and inactivity of the sex chromosomes in the flour moth Ephestia kuehniella and the silkworm Bombyx mori, using immunostaining with anti-H3K9me2/3, anti-RNA polymerase II, and fluoro-uridine (FU) labelling of nascent transcripts, with conventional widefield fluorescence microscopy and 'spatial structured illumination microscopy' (3D-SIM). The Z chromosome is euchromatic in somatic cells and throughout meiosis. It is transcriptionally active in somatic cells and in the postpachaytene stage of meiosis. The W chromosome in contrast is heterochromatic in somatic cells as well as in meiotic cells at pachytene, but euchromatic and transcriptionally active like all other chromosomes at postpachytene. As the W chromosomes are apparently devoid of protein-coding genes, their transcripts must be non-coding. We found no indication of 'meiotic sex chromosome inactivation' (MSCI) in the two species. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2020 Elektronická adresa https://link.springer.com/article/10.1007%2Fs00412-019-00722-8
Počet záznamů: 1