Počet záznamů: 1
Hypoxia favors myosin heavy chain beta gene expression in an Hif-1alpha-dependent manner
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SYSNO ASEP 0506842 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Hypoxia favors myosin heavy chain beta gene expression in an Hif-1alpha-dependent manner Tvůrce(i) Binó, Lucia (BFU-R) ORCID
Procházková, Jiřina (BFU-R) RID, ORCID
Radaszkiewicz, K. A. (CZ)
Kučera, J. (CZ)
Kudová, Jana (BFU-R) ORCID
Pachernik, J. (CZ)
Kubala, Lukáš (BFU-R) RID, ORCIDCelkový počet autorů 7 Zdroj.dok. OncoTarget. - : Impact Journals LLC - ISSN 1949-2553
Roč. 8, č. 48 (2017), s. 83684-83697Poč.str. 14 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova inducible factor ; mammalian target ; human heart ; myocardial-ischemia Vědní obor RIV FD - Onkologie a hematologie Obor OECD Oncology CEP LD11015 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora BFU-R - RVO:68081707 UT WOS 000413030900026 DOI 10.18632/oncotarget.19016 Anotace The potentiation of the naturally limited regenerative capacity of the heart is dependent on an understanding of the mechanisms that are activated in response to pathological conditions such as hypoxia. Under these conditions, the expression of genes suggested to support cardiomyocyte survival and heart adaptation is triggered. Particularly important are changes in the expression of myosin heavy chain (MHC) isoforms. We propose here that alterations in the expression profiles of MHC genes are induced in response to hypoxia and are primarily mediated by hypoxia inducible factor (HIF). In in vitro models of mouse embryonic stem cell-derived cardiomyocytes, we showed that hypoxia (1% O-2) or the pharmacological stabilization of HIFs significantly increased MHCbeta (Myh7) gene expression. The key role of HIF-1alpha is supported by the absence of these effects in HIF-1alpha-deficient cells, even in the presence of HIF-2alpha. Interestingly, ChIP analysis did not confirm the direct interaction of HIF-1alpha with putative HIF response elements predicted in the MHCalpha and beta encoding DNA region. Further analyses showed the significant effect of the mTOR signaling inhibitor rapamycin in inducing Myh7 expression and a hypoxia-triggered reduction in the levels of antisense RNA transcripts associated with the Myh7 gene locus. Overall, the recognized and important role of HIF in the regulation of heart regenerative processes could be highly significant for the development of novel therapeutic interventions in heart failure. Pracoviště Biofyzikální ústav Kontakt Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Rok sběru 2020 Elektronická adresa http://www.oncotarget.com/index.php?journal=oncotarget&page=article&op=download&path%5B%5D=19016&path%5B%5D=60953
Počet záznamů: 1