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The use of 1,5-diaminonaphthalene for matrix-assisted laser desorption/ionization mass spectrometry imaging of brain in neurodegenerative disorders
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SYSNO ASEP 0506829 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název The use of 1,5-diaminonaphthalene for matrix-assisted laser desorption/ionization mass spectrometry imaging of brain in neurodegenerative disorders Tvůrce(i) Strnad, Štěpán (UOCHB-X) ORCID
Pražienková, Veronika (UOCHB-X) RID, ORCID
Sýkora, D. (CZ)
Cvačka, Josef (UOCHB-X) RID, ORCID
Maletínská, Lenka (UOCHB-X) RID, ORCID
Popelová, Andrea (UOCHB-X) RID, ORCID
Vrkoslav, Vladimír (UOCHB-X) RID, ORCIDZdroj.dok. Talanta. - : Elsevier - ISSN 0039-9140
Roč. 201, Aug 15 (2019), s. 364-372Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova 1,5-diaminonaphthalene ; ImagePrep ; iMatrixSpray ; mass spectrometry imaging ; sublimation ; mouse model of neurodegeneration Vědní obor RIV CB - Analytická chemie, separace Obor OECD Analytical chemistry CEP EF16_019/0000729 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GA16-00918S GA ČR - Grantová agentura ČR Způsob publikování Omezený přístup Institucionální podpora UOCHB-X - RVO:61388963 UT WOS 000471084300049 EID SCOPUS 85064320719 DOI 10.1016/j.talanta.2019.03.117 Anotace The selection of a suitable matrix and deposition technique constitutes a critical step in successful matrix-assisted laser desorption/ionization mass spectrometry imaging measurement. In the present work, we compared three techniques of matrix deposition, specifically, sublimation and spraying of 1,5-diaminonaphthalene with two automatic sprayers, ImagePrep and iMatrixSpray. The studied methods were evaluated in experiments for the analysis of lipid composition in the brains of two mouse models of neurodegeneration: APP/PS1 mice with plaques of amyloid beta(A beta) peptides and THY-Tau22 mice with pathologically hyperphosphorylated Tau protein, two hallmarks of Alzheimer's disease-like pathology. The sublimation method provided irreproducible results because of significant matrix loss due to the high vacuum in the ion source and laser irradiation. In contrast, the ImagePrep and iMatrixSpray provided stable film of the matrix. The deposited matrix was stable during the measurement, and highly reproducible datasets were obtained. Both spraying methods yielded similar results with approximately the same number of detected lipids and comparable signal intensity. However, iMatrixSpray has two main advantages: a faster matrix deposition and the formation of smaller matrix crystals leading to better spatial resolution. In the APP/PS1 mouse model at an age of 6 months, we found colocalization of A beta plaques with different phospholipids, sphingolipids and lysophospholipids. We did not find a difference in lipid composition between the THY-Tau22 mice and the wild-type controls. The results indicate that hyperpho-sphorylation of tau protein in the THY-Tau22 mouse model at the age of 6 months is not accompanied with a significant change in lipid content in the brain. However, considering limitations of the used method, a definitive conclusion in this respect will need further research. Pracoviště Ústav organické chemie a biochemie Kontakt asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434 Rok sběru 2020 Elektronická adresa https://www.sciencedirect.com/science/article/abs/pii/S0039914019303856?via%3Dihub
Počet záznamů: 1