Počet záznamů: 1

P2X2 Receptor Expression and Function Is Upregulated in the Rat Supraoptic Nucleus Stimulated Through Refeeding After Fasting

SYSNO ASEP	0506583
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	P2X2 Receptor Expression and Function Is Upregulated in the Rat Supraoptic Nucleus Stimulated Through Refeeding After Fasting
Tvůrce(i)	Ivetic, Milorad (FGU-C)_{RID, ORCID} Bhattacharya, Anirban (FGU-C)_{RID, ORCID, SAI} Zemková, Hana (FGU-C)_{RID, ORCID}
Číslo článku	284
Zdroj.dok.	Frontiers in Cellular Neuroscience. - : Frontiers Media Roč. 13, Jun 26 (2019)
Poč.str.	13 s.
Jazyk dok.	eng - angličtina
Země vyd.	CH - Švýcarsko
Klíč. slova	supraoptic nucleus ; P2X receptor ; P2Y receptor ; ATP ; GABA ; arginine vasopressin ; oxytocin
Vědní obor RIV	FH - Neurologie, neurochirurgie, neurovědy
Obor OECD	Neurosciences (including psychophysiology
CEP	LQ1604 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
	GA16-12695S GA ČR - Grantová agentura ČR
	ED1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
	GA18-05413S GA ČR - Grantová agentura ČR
Způsob publikování	Open access
Institucionální podpora	FGU-C - RVO:67985823
UT WOS	000473163400001
EID SCOPUS	85068452024
DOI	10.3389/fncel.2019.00284
Anotace	Magnocellular neurons in the supraoptic nucleus (SON), which synthesize and release arginine vasopressin (AVP) and oxytocin (OT), express several subtypes of ATP-stimulated purinergic P2X receptors (P2XR) that modulate neuronal activity as well as neurotransmitter and hormone release. However, the physiological impact of this modulation is not well understood. Here, we tested a hypothesis that P2XRs play a role in the sustained release of hormones from SON neurons stimulated through fasting/refeeding. We studied the effect of 2 h of refeeding after 48 h of fasting on P2XR and P2YR mRNA expression and ATP-induced presynaptic and postsynaptic responses in the SON of 30-day-old rats. Quantitative real-time PCR revealed that the expression of P2X2R and AVP mRNA was upregulated, whereas P2X4R, P2X7R, P2Y2R, and OT mRNA levels were not significantly changed and P2Y1R mRNA expression was decreased. Whole-cell patch clamp recordings performed on isolated rat brain slices showed that the amplitude of the ATP-stimulated somatic current and the ATP-induced increases in the frequency of spontaneous GABAergic inhibitory postsynaptic currents were significantly higher in SON neurons from fasted/refed rats than in SON neurons from normally fed rats. No evidence was found for changes in the presynaptic effect of ATP in SON neurons not expressing somatic P2XRs. These results suggest that the increased activity of SON neurons synthesizing AVP is associated with enhanced expression of P2X2Rs on neuronal cell bodies and their GABAergic presynaptic nerve terminals.
Pracoviště	Fyziologický ústav
Kontakt	Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
Rok sběru	2020
Elektronická adresa	https://doi.org/10.3389/fncel.2019.00284

Počet záznamů: 1