Počet záznamů: 1
Microfluidic-assisted engineering of quasi-monodisperse pH-responsive polymersomes toward advanced platforms for the intracellular delivery of hydrophilic therapeutics
- 1.
SYSNO ASEP 0505875 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Microfluidic-assisted engineering of quasi-monodisperse pH-responsive polymersomes toward advanced platforms for the intracellular delivery of hydrophilic therapeutics Tvůrce(i) Calumby Albuquerque, Lindomar J. (UMCH-V)
Sincari, Vladimir (UMCH-V) ORCID, RID
Jäger, Alessandro (UMCH-V) RID, ORCID
Konefal, Rafal (UMCH-V) RID, ORCID
Pánek, Jiří (UMCH-V) RID, ORCID
Černoch, Peter (UMCH-V) RID, ORCID
Pavlova, Ewa (UMCH-V) RID
Štěpánek, Petr (UMCH-V) RID, ORCID
Giacomelli, F. C. (BR)
Jäger, Eliezer (UMCH-V) ORCID, RIDZdroj.dok. Langmuir. - : American Chemical Society - ISSN 0743-7463
Roč. 35, č. 25 (2019), s. 8363-8372Poč.str. 10 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova block copolymers ; microfluidics ; self-assembled Vědní obor RIV CD - Makromolekulární chemie Obor OECD Polymer science CEP GA17-09998S GA ČR - Grantová agentura ČR TN01000008 GA TA ČR - Technologická agentura ČR 8J18FR038 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Omezený přístup Institucionální podpora UMCH-V - RVO:61389013 UT WOS 000473248000020 EID SCOPUS 85067954153 DOI 10.1021/acs.langmuir.9b01009 Anotace The extracellular and subcellular compartments are characterized by specific pH levels that can be modified by pathophysiological states. This scenario encourages the use of environmentally responsive nanomedicines for the treatment of damaged cells. We have engineered doxorubicin (DOX)-loaded pH-responsive polymersomes using poly([N-(2-hydroxypropyl)]methacrylamide)-b-poly[2-(diisopropylamino)ethyl methacrylate] block copolymers (PHPMAm-b-PDPAn). We demonstrate that, by taking advantage of the microfluidic technology, quasi-monodisperse assemblies can be created. This feature is of due relevance because highly uniform nanoparticles commonly exhibit more consistent biodistribution and cellular uptake. We also report that the size of the polymer vesicles can be tuned by playing with the inherent mechanical parameters of the microfluidic protocol. This new knowledge can be used to engineer size-specific nanomedicines for enhanced tumor accumulation if the manufacturing is performed with previous knowledge of tumor characteristics (particularly the degree of vascularity and porosity). The pH-dependent DOX release was further investigated evidencing the ability of polymersome to sustain encapsulated hydrophilic molecules when circulating in physiological environment (pH 7.4). This suggests nonrelevant drug leakage during systemic circulation. On the other hand, polymersome disassembly in slightly acid environments takes place enabling fast DOX release, thereby making the colloidal carriers highly cytotoxic. These features encourage the use of such advanced pH-responsive platforms to target damaged cells while preserving healthy environments during systemic circulation. Pracoviště Ústav makromolekulární chemie Kontakt Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Rok sběru 2020 Elektronická adresa https://pubs.acs.org/doi/10.1021/acs.langmuir.9b01009
Počet záznamů: 1