Počet záznamů: 1
Multifaceted activity of cytokinin in leaf development shapes its size and structure in Arabidopsis
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SYSNO ASEP 0504121 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Multifaceted activity of cytokinin in leaf development shapes its size and structure in Arabidopsis Tvůrce(i) Skalák, J. (CZ)
Vercruyssen, L. (BE)
Claeys, H. (BE)
Hradilová, J. (CZ)
Černý, M. (CZ)
Novák, Ondřej (UEB-Q) RID, ORCID, SAI
Plačková, Lenka (UEB-Q) ORCID, RID
Saiz-Fernández, I. (CZ)
Skaláková, P. (CZ)
Coppens, F. (CZ)
D'hondt, B. (BE)
Koukalová, Š. (CZ)
Zouhar, J. (CZ)
Inzé, D. (BE)
Brzobohatý, Břetislav (BFU-R) RID, ORCIDCelkový počet autorů 15 Zdroj.dok. Plant Journal. - : Wiley - ISSN 0960-7412
Roč. 97, č. 5 (2019), s. 805-824Poč.str. 20 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova Arabidopsis thaliana ; cytokinin ; leaf development ; proteome ; transcriptome Vědní obor RIV ED - Fyziologie Obor OECD Biochemical research methods Vědní obor RIV – spolupráce Biofyzikální ústav - Botanika CEP GA17-04607S GA ČR - Grantová agentura ČR GA18-07563S GA ČR - Grantová agentura ČR LQ1601 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy EF16_019/0000738 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UEB-Q - RVO:61389030 ; BFU-R - RVO:68081707 UT WOS 000462684900002 EID SCOPUS 85062641549 DOI 10.1111/tpj.14285 Anotace The phytohormone cytokinin has been shown to affect many aspects of plant development ranging from the regulation of the shoot apical meristem to leaf senescence. However, some studies have reported contradictory effects of cytokinin on leaf physiology. Therefore cytokinin treatments cause both chlorosis and increased greening and both lead to decrease or increase in cell size. To elucidate this multifaceted role of cytokinin in leaf development, we have employed a system of temporal controls over the cytokinin pool and investigated the consequences of modulated cytokinin levels in the third leaf of Arabidopsis. We show that, at the cell proliferation phase, cytokinin is needed to maintain cell proliferation by blocking the transition to cell expansion and the onset of photosynthesis. Transcriptome profiling revealed regulation by cytokinin of a gene suite previously shown to affect cell proliferation and expansion and thereby a molecular mechanism by which cytokinin modulates a molecular network underlying the cellular responses. During the cell expansion phase, cytokinin stimulates cell expansion and differentiation. Consequently, a cytokinin excess at the cell expansion phase results in an increased leaf and rosette size fueled by higher cell expansion rate, yielding higher shoot biomass. Proteome profiling revealed the stimulation of primary metabolism by cytokinin, in line with an increased sugar content that is expected to increase turgor pressure, representing the driving force of cell expansion. Therefore, the developmental timing of cytokinin content fluctuations, together with a tight control of primary metabolism, is a key factor mediating transitions from cell proliferation to cell expansion in leaves. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2020 Elektronická adresa http://doi.org/10.1111/tpj.14285
Počet záznamů: 1