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Kocuria Bacterial Isolates from Radioactive Springs of Jachymov spa (Joachimsthal) as Sources of Polyunsaturated Fatty Acids
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SYSNO ASEP 0503944 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Kocuria Bacterial Isolates from Radioactive Springs of Jachymov spa (Joachimsthal) as Sources of Polyunsaturated Fatty Acids Tvůrce(i) Řezanka, Tomáš (MBU-M) ORCID
Gharwalová, L. (CZ)
Nováková, G. (CZ)
Kolouchová, I. (CZ)
Uhlík, O. (CZ)
Sigler, Karel (MBU-M) RIDZdroj.dok. Lipids. - : Wiley - ISSN 0024-4201
Roč. 54, 2-3 (2019), s. 177-187Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova Arachidonic and eicosapentaenoic acids ; Kocuria sp ; Polyunsaturated fatty acids Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology CEP GA17-00027S GA ČR - Grantová agentura ČR Způsob publikování Omezený přístup Institucionální podpora MBU-M - RVO:61388971 UT WOS 000462095000006 EID SCOPUS 85062794497 DOI 10.1002/lipd.12136 Anotace Four bacterial isolates, which produced polyunsaturated fatty acids (PUFA), were isolated from water samples of radioactive springs collected from Jachymov spa. Jachymov (Sankt Joachimsthal) is a city in northwestern Bohemia, where Marie and Pierre Curie isolated radium in 1898 from the mineral uraninite. To date, four springs (Agricola, Behounek, C1, and Curie) have been used for spa purposes, that is for the treatment of nervous and rheumatic disorders by constantly produced radioactive gas radon (Rn-222) dissolved in the water. The radioactivity reaches 24 kBq/L. Using 16S rRNA gene sequence analysis, all four isolates were identified as members of the genus Kocuria, with two isolates designated 208 and 401 affiliated with Kocuria kristinae, while isolates 101 and 301 most likely with K. rhizophila. The content of fatty acids in polar lipids was determined by gas chromatography-mass spectrometry (GC-MS) and two PUFA, that is arachidonic and eicosapentaenoic, were identified. The position of double bonds was confirmed by GC-MS of 3-pyridylcarbinol (formerly picolinyl) esters. We assume that all four isolates of Kocuria produce PUFA to increase the stability of cell membranes, which may be impaired by the reaction of the reactive oxygen species. These can arise, for example, because of radiation during Rn-222 decay. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2020 Elektronická adresa https://aocs.onlinelibrary.wiley.com/doi/abs/10.1002/lipd.12136
Počet záznamů: 1