Počet záznamů: 1
Agrobacterium rhizogenes-mediated transformation of a dioecious plant model Silene latifolia
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SYSNO ASEP 0502573 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Agrobacterium rhizogenes-mediated transformation of a dioecious plant model Silene latifolia Tvůrce(i) Hudzieczek, Vojtěch (BFU-R) ORCID
Čegan, Radim (BFU-R) RID, ORCID
Čermak, T. (US)
Bačovská, Nela (BFU-R)
Machálková, Zuzana (BFU-R)
Doležal, Karel (UEB-Q) RID, ORCID
Plíhalová, Lucie (UEB-Q) ORCID, RID
Voytas, D.F. (US)
Hobza, Roman (BFU-R) RID, ORCID
Vyskot, Boris (BFU-R) RID, ORCIDCelkový počet autorů 10 Zdroj.dok. New Biotechnology. - : Elsevier - ISSN 1871-6784
Roč. 48, JAN 25 2019 (2019), s. 20-28Poč.str. 9 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova y-chromosome ; genetic-transformation ; biological-activity Vědní obor RIV CE - Biochemie Obor OECD Biochemistry and molecular biology Vědní obor RIV – spolupráce Ústav experimentální botaniky - Biochemie CEP GA17-00567S GA ČR - Grantová agentura ČR LO1204 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora BFU-R - RVO:68081707 ; UEB-Q - RVO:61389030 UT WOS 000450366200004 DOI 10.1016/j.nbt.2018.04.001 Anotace Silene latifolia serves as a model species to study dioecy, the evolution of sex chromosomes, dosage compensation and sex-determination systems in plants. Currently, no protocol for genetic transformation is available for this species, mainly because S. latifolia is considered recalcitrant to in vitro regeneration and infection with Agrobacterium tumefaciens. Using cytokinins and their synthetic derivatives, we markedly improved the efficiency of regeneration. Several agrobacterial strains were tested for their ability to deliver DNA into S. latifolia tissues leading to transient and stable expression of the GUS reporter. The use of Agrobacterium rhizogenes strains resulted in the highest transformation efficiency (up to 4.7% of stable transformants) in hairy root cultures. Phenotypic and genotypic analyses of the T1 generation suggested that the majority of transformation events contain a small number of independent T-DNA insertions and the transgenes are transmitted to the progeny in a Mendelian pattern of inheritance. In short, we report an efficient and reproducible protocol for leaf disc transformation and subsequent plant regeneration in S. latifolia, based on the unique combination of infection with A. rhizogenes and plant regeneration from hairy root cultures using synthetic cytokinins. A protocol for the transient transformation of S. latifolia protoplasts was also developed and applied to demonstrate the possibility of targeted mutagenesis of the sex linked gene SlAP3 by TALENs and CRISPR/Cas9. Pracoviště Biofyzikální ústav Kontakt Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Rok sběru 2019 Elektronická adresa https://doi.org/10.1016/j.nbt.2018.04.001
Počet záznamů: 1