Počet záznamů: 1

Up-regulation of mu-, delta- and kappa-opioid receptors in concanavalin A-stimulated rat spleen lymphocytes

SYSNO ASEP	0501918
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Up-regulation of mu-, delta- and kappa-opioid receptors in concanavalin A-stimulated rat spleen lymphocytes
Tvůrce(i)	Cechová, Kristína (FGU-C)_{RID, ORCID} Hloušková, Martina (FGU-C) Javorková, Eliška (UEM-P)_RID Roubalová, Lenka (FGU-C)_{RID, ORCID, SAI} Ujčíková, Hana (FGU-C)_{RID, ORCID} Holáň, Vladimír (UEM-P)_RID Svoboda, Petr (FGU-C)_{RID, ORCID}
Zdroj.dok.	Journal of Neuroimmunology. - : Elsevier - ISSN 0165-5728 Roč. 321, Aug 15 (2018), s. 12-23
Poč.str.	12 s.
Jazyk dok.	eng - angličtina
Země vyd.	NL - Nizozemsko
Klíč. slova	opioids receptors ; up-regulation ; concanavalin A ; rat spleen lymphocytes ; G proteins ; beta-arrestin-1/2
Vědní obor RIV	ED - Fyziologie
Obor OECD	Physiology (including cytology)
Vědní obor RIV – spolupráce	Ústav experimentální medicíny - Morfologické obory a cytologie
CEP	GA17-07070S GA ČR - Grantová agentura ČR
	GA17-05903S GA ČR - Grantová agentura ČR
Institucionální podpora	FGU-C - RVO:67985823 ; UEM-P - RVO:68378041
UT WOS	000438322000002
EID SCOPUS	85052665655
DOI	10.1016/j.jneuroim.2018.05.008
Anotace	Regulation of mu-, delta- and kappa-opioid receptor protein level in spleen lymphocytes when stimulated by mitogen is not known. To answer the question whether these cells do express opioid receptor (OR) proteins, primary, fresh rat spleen lymphocytes were prepared and stimulated for 48 h with mitogenic dose of Con A. The unstimulated lymphocytes did not express mu- and delta-OR proteins in detectable amounts, however, stimulation with Con A resulted in appearance of clearly detectable immunoblot signals of both mu-OR and delta-OR. kappa-OR were detected already in primary cells and increased 2.4-fold in Con A-stimulated cells. These results were supported by data obtained by flow cytometry analysis indicating a dramatic increase in number of mu-, delta- and kappa-OR expressing cells after mitogen stimulation. The newly synthesized mu-, delta- and kappa-OR in Con A-stimulated spleen lymphocytes were present in the cells interior and not functionally mature, at least in terms of their ability to enhance activity of trimeric G proteins determined by three different protocols of agonist-stimulated, high-affinity [S-35]GTP gamma S binding assay. The up-regulation of mu-, delta- and kappa-OR was associated with specific decrease of their cognate trimeric G proteins, G(i)1 alpha/G(i)2 alpha, the other G alpha and G beta subunits were unchanged. The level of beta-arrestin-1/2 was also decreased in Con A-stimulated splenocytes. We conclude that up-regulation of OR expression level in spleen lymphocytes by Con A proceeds in conjunction with down-regulation of their intracellular signaling partners, G(i)1 alpha/G(i)2 alpha proteins and beta-arrestin-1/2. These regulatory proteins are expressed in high amounts already in unstimulated cells and decreased by mitogen stimulation.
Pracoviště	Fyziologický ústav
Kontakt	Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
Rok sběru	2019

Počet záznamů: 1