Počet záznamů: 1
Probing the Thermodynamics of Incorporation of N-6-methyl-dATP Opposite an Abasic Site, dCMP, and dTMP During Simulated DNA Synthesis by Differential Scanning Calorimetry
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SYSNO ASEP 0501578 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Probing the Thermodynamics of Incorporation of N-6-methyl-dATP Opposite an Abasic Site, dCMP, and dTMP During Simulated DNA Synthesis by Differential Scanning Calorimetry Tvůrce(i) Malina, Jaroslav (BFU-R) ORCID
Brabec, Viktor (BFU-R) RID, ORCIDCelkový počet autorů 2 Zdroj.dok. ChemistrySelect. - : Wiley - ISSN 2365-6549
Roč. 3, č. 46 (2018), s. 13076-13080Poč.str. 5 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova polymerase ; damage ; nucleoside ; energetics Vědní obor RIV CE - Biochemie Obor OECD Biochemistry and molecular biology CEP GA17-09436S GA ČR - Grantová agentura ČR Institucionální podpora BFU-R - RVO:68081707 UT WOS 000453576900014 DOI 10.1002/slct.201803565 Anotace Previous reports indicated that when an abasic (apurinic/apyrimidinic, AP) site is bypassed by DNA polymerases, dATP is preferentially inserted. Here we evaluate, using differential scanning calorimetry, the thermodynamic changes associated with incorporation of N-6-methyl-dATP opposite an AP site, dCMP, and dTMP during simulated DNA polymerization. The results confirm that AP sites block DNA polymerases one nucleotide prior to the lesion. Thermodynamic data imply that the propensity of N-6-methyl-dAMP for elongation, when incorporated opposite an AP site, is higher than that of dAMP in agreement with a higher promutagenic potential of N-6-methyl-dATP if placed opposite a non-instructional DNA lesion, such as an AP site. Pracoviště Biofyzikální ústav Kontakt Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Rok sběru 2019
Počet záznamů: 1