Počet záznamů: 1

Poly(D,L-lactide)/polyethylene glycol micro/nanofiber mats as paclitaxel-eluting carriers: preparation and characterization of fibers, in vitro drug release, antiangiogenic activity and tumor recurrence prevention

SYSNO ASEP	0500120
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Poly(D,L-lactide)/polyethylene glycol micro/nanofiber mats as paclitaxel-eluting carriers: preparation and characterization of fibers, in vitro drug release, antiangiogenic activity and tumor recurrence prevention
Tvůrce(i)	Hobzová, Radka (UMCH-V)_{RID, ORCID} Hampejsová, Z. (CZ) Černá, T. (CZ) Hraběta, J. (CZ) Venclíková, Kristýna (UMCH-V)_RID Jedelská, J. (DE) Bakowsky, U. (DE) Bosáková, Z. (CZ) Lhotka, M. (CZ) Vaculín, Š. (CZ) Franěk, M. (CZ) Steinhart, Miloš (UMCH-V)_RID Kovářová, Jana (UMCH-V)_RID Michálek, Jiří (UMCH-V)_{RID, ORCID} Širc, Jakub (UMCH-V)_{RID, ORCID}
Zdroj.dok.	Materials Science & Engineering C-Materials for Biological Applications. - : Elsevier - ISSN 0928-4931 Roč. 98, May (2019), s. 982-993
Poč.str.	12 s.
Jazyk dok.	eng - angličtina
Země vyd.	NL - Nizozemsko
Klíč. slova	PLA/PEG micro/nanofibers ; needleless electrospinning ; paclitaxel quantification
Vědní obor RIV	CD - Makromolekulární chemie
Obor OECD	Polymer science
CEP	GA16-04863S GA ČR - Grantová agentura ČR
	LQ1604 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
	ED1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
Způsob publikování	Omezený přístup
Institucionální podpora	UMCH-V - RVO:61389013
UT WOS	000461526800097
EID SCOPUS	85060105949
DOI	10.1016/j.msec.2019.01.046
Anotace	Poly(d,l-lactide)/polyethylene glycol (PLA/PEG) micro/nanofibers loaded with paclitaxel (PTX, 10 wt%) were prepared by needless electrospinning technology, which allows large scale production for real medicinal practice. The fiber structure and properties were investigated by several methods including scanning electron microscopy, nitrogen adsorption/desorption isotherm measurements, differential scanning calorimetry, and X-ray diffraction measurements to examine their morphology (fiber diameter distribution, specific surface area, and total pore volume), composition, drug-loading efficiency, and physical state. An HPLC-UV method was optimized and validated to quantify in vitro PTX release into PBS. The results showed that the addition of PEG into PLA fibers promoted the release of higher amounts of hydrophobic PTX over prolonged time periods compared to fibers without PEG. An in vitro cell assay demonstrated the biocompatibility of PLA/PEG fibrous materials and showed significant cytotoxicity of PTX-loaded PLA/PEG fibers against a human fibrosarcoma HT1080 cell line. The chick chorioallantoic membrane assay proved that PTX-loaded fibers exhibited antiangiogenic activity, with a pronounced effect in the case of the PEG-containing fibers. In vivo evaluation of PTX-loaded PLA/PEG fibers in a human fibrosarcoma recurrence model showed statistically significant inhibition in tumor incidence and growth after primary tumor resection compared to other treatment groups.
Pracoviště	Ústav makromolekulární chemie
Kontakt	Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358
Rok sběru	2020
Elektronická adresa	https://www.sciencedirect.com/science/article/pii/S0928493118308683?via%3Dihub

Počet záznamů: 1