Počet záznamů: 1
Metabarcoding analysis of strongylid nematode diversity in two sympatric primate species
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SYSNO ASEP 0498784 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Metabarcoding analysis of strongylid nematode diversity in two sympatric primate species Tvůrce(i) Pafčo, B. (CZ)
Čížková, D. (CZ)
Kreisinger, J. (CZ)
Hasegawa, H. (JP)
Vallo, P. (CZ)
Shutt, K. (GB)
Todd, A. (CF)
Petrželková, Klára Judita (BC-A) RID
Modrý, David (BC-A) RID, ORCIDCelkový počet autorů 9 Číslo článku 5933 Zdroj.dok. Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 8, APR 12 (2018)Poč.str. 11 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova WESTERN LOWLAND GORILLAS ; OESOPHAGOSTOMUM-BIFURCUM NEMATODA ; INTERNAL TRANSCRIBED SPACER ; NECATOR-AMERICANUS Vědní obor RIV GJ - Choroby a škůdci zvířat, veterinární medicína Obor OECD Veterinary science Institucionální podpora BC-A - RVO:60077344 UT WOS 000429785900073 EID SCOPUS 85045440671 DOI https://doi.org/10.1038/s41598-018-24126-3 Anotace Strongylid nematodes in large terrestrial herbivores such as great apes, equids, elephants, and humans tend to occur in complex communities. However, identification of all species within strongylid communities using traditional methods based on coproscopy or single nematode amplification and sequencing is virtually impossible. High-throughput sequencing (HTS) technologies provide opportunities to generate large amounts of sequence data and enable analyses of samples containing a mixture of DNA from multiple species/genotypes. We designed and tested an HTS approach for strain-level identification of gastrointestinal strongylids using ITS-2 metabarcoding at the MiSeq Illumina platform in samples from two free-ranging non-human primate species inhabiting the same environment, but differing significantly in their host traits and ecology. Although we observed overlapping of particular haplotypes, overall the studied primate species differed in their strongylid nematode community composition. Using HTS, we revealed hidden diversity in the strongylid nematode communities in non-human primates, more than one haplotype was found in more than 90% of samples and coinfections of more than one putative species occurred in 80% of samples. In conclusion, the HTS approach on strongylid nematodes, preferably using fecal samples, represents a time and cost-efficient way of studying strongylid communities and provides a resolution superior to traditional approaches. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2019 Elektronická adresa https://www.nature.com/articles/s41598-018-24126-3
Počet záznamů: 1