Počet záznamů: 1  

Metabarcoding analysis of strongylid nematode diversity in two sympatric primate species

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    SYSNO ASEP0498784
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevMetabarcoding analysis of strongylid nematode diversity in two sympatric primate species
    Tvůrce(i) Pafčo, B. (CZ)
    Čížková, D. (CZ)
    Kreisinger, J. (CZ)
    Hasegawa, H. (JP)
    Vallo, P. (CZ)
    Shutt, K. (GB)
    Todd, A. (CF)
    Petrželková, Klára Judita (BC-A) RID
    Modrý, David (BC-A) RID
    Celkový počet autorů9
    Číslo článku5933
    Zdroj.dok.Scientific Reports - ISSN 2045-2322
    Roč. 8, APR 12 (2018)
    Poč.str.11 s.
    Forma vydáníOnline - E
    Jazyk dok.eng - angličtina
    Země vyd.GB - Velká Británie
    Klíč. slovaWESTERN LOWLAND GORILLAS ; OESOPHAGOSTOMUM-BIFURCUM NEMATODA ; INTERNAL TRANSCRIBED SPACER ; NECATOR-AMERICANUS
    Vědní obor RIVGJ - Choroby a škůdci zvířat, veterinární medicína
    Obor OECDVeterinary science
    Institucionální podporaBC-A - RVO:60077344
    UT WOS000429785900073
    EID SCOPUS85045440671
    DOI10.1038/s41598-018-24126-3
    AnotaceStrongylid nematodes in large terrestrial herbivores such as great apes, equids, elephants, and humans tend to occur in complex communities. However, identification of all species within strongylid communities using traditional methods based on coproscopy or single nematode amplification and sequencing is virtually impossible. High-throughput sequencing (HTS) technologies provide opportunities to generate large amounts of sequence data and enable analyses of samples containing a mixture of DNA from multiple species/genotypes. We designed and tested an HTS approach for strain-level identification of gastrointestinal strongylids using ITS-2 metabarcoding at the MiSeq Illumina platform in samples from two free-ranging non-human primate species inhabiting the same environment, but differing significantly in their host traits and ecology. Although we observed overlapping of particular haplotypes, overall the studied primate species differed in their strongylid nematode community composition. Using HTS, we revealed hidden diversity in the strongylid nematode communities in non-human primates, more than one haplotype was found in more than 90% of samples and coinfections of more than one putative species occurred in 80% of samples. In conclusion, the HTS approach on strongylid nematodes, preferably using fecal samples, represents a time and cost-efficient way of studying strongylid communities and provides a resolution superior to traditional approaches.
    PracovištěBiologické centrum (od r. 2006)
    KontaktDana Hypšová, eje@eje.cz, Tel.: 387 775 214
    Rok sběru2019
    Elektronická adresahttps://www.nature.com/articles/s41598-018-24126-3
Počet záznamů: 1